BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516
Home -> Cell Biology -> Exosomes -> Exosome Lipidomics and Metabolomics Service

Exosome Lipidomics and Metabolomics Service

For Deeper Insights from Exosomes

• Discover novel circulating biomarkers
• Learn more about exosome biology
• Send your samples to BioCat Heidelberg and receive data in 5 - 6 weeks


Whether you’re interested in circulating biomarker discovery, basic exosome research, or other exosome-related studies, SBI’s Exosome Lipidomics & Metabolomics Service helps you quickly and efficiently get the most information from your exosomes. Simply send us your sample or purified exosomes and we’ll send back a spreadsheet with putative identifications, mass/charge ratios, and differential analysis (if requested).

What can lipidomics of exosomes tell you?

Lipids are an important part of cellular physiology, and are increasingly being recognized for their importance in exosome biology as well. Exosomes were recently shown to have the highest lipid-to-protein ratio of all classes of extracellular vesicles (1), with lipid content that both differs from the parent cell the vesicles are shed from (2) and also changes as exosomes undergo a variety of physiological processes (3). These unique lipid profiles can serve as novel circulating biomarkers, and recent evidence suggests that specific lipid species carried by exosomes can also modulate the function of recipient cells (4).

With so much information revealed by lipid content, lipidomics studies of exosomes are a great way to identify lipid-based biomarkers and for understanding vesicle biogenesis and function (5).


What can metabolomics of exosomes tell you?

In addition to lipids, exosomes carry a wide range of metabolites that can also be used for biomarker discovery. A recent study found distinctly different populations of metabolites in PANC1 cells treated with TGF-b, a known driver of cancer progression, versus a control group, suggesting that metabolite profiling can be used for differentiating cellular states (6). Like exosomal lipids, exosomal metabolites can also take an active role in the target cell, and have been shown to reprogram metabolic machinery upon uptake by cancer cells, fueling growth (7).


Service Description

The standard service (Catalog no. CSEQ850A-1-SBI) offers UPLC-FTMS analysis of both very polar metabolites in the aqueous phase and lipids and other non-polar metabolites in the organic phase. Individual polar or non-polar analysis are also available.

Input sample requirements:

Serum 500 ul — 1ml
Plasma 500 ul — 1ml
Cell Media 5 ml — 10ml
Urine 5ml — 10ml
Spinal Fluid 5ml — 10ml
Ascites Fluid 500 ul — 1ml
Other: Please inquire

Data deliverables (typical time-line: 5-6 weeks):

• MS data will be delivered as excel files with putative identification based on the m/z ratio of the analytes
• Differential analysis of lipids and metabolites between treatment groups will be included in the excel files

Note that all IDs are based only on retention time, accurate mass measurement, and preliminary MS/MS data, and cannot be guaranteed. Not all peaks will be identified, and we recommend that any peaks of interest—either due to the identity of the compound reported and/or change in intensity between samples—MUST be verified by additional targeted analysis.


Example data:

Exosomes were harvested from 300 uL of serum of a healthy human subject and a subject with Acute Myeloid Leukemia (AML). Discovery-based lipidomics /metabolomics profiling was performed on the serum vesicles from the two subjects.

Image


Related Links

Ask for a quotation (bulk discounts will apply)


PDF-Downloads - Will open in new browser window

Exosome Lipidomics and Metabolomics Brochure

 

 

Description Cat# Size Price    
Exosome Lipidomics & Metabolomics Service - Complete (Polar and Non-Polar) CSEQ850A-1-SBI Mass Spectrometry Data and Analysis 2497 € DETAILS   Add to Cart 
Exosome Lipidomics & Metabolomics Service - Polar only CSEQ851A-1-SBI Mass Spectrometry Data and Analysis 2010 € DETAILS   Add to Cart 
Exosome Lipidomics & Metabolomics Service - Non-Polar only CSEQ852A-1-SBI Mass Spectrometry Data and Analysis 2010 € DETAILS   Add to Cart 

BioCat Autumn Special

Autumn Special

10% OFF Stellaris RNA FISH Probe Sets & Buffers

50 Euro Voucher

Email Newsletter

Subscribe to the BioCat Email Newsletter and receive a 50 € voucher.

Cell-based Assays

Cell-based assays

Choose from a wide variety of cell-based assays designed for greater accuracy and increased throughput.

50% OFF Fluorescent Proteins

Fluorescent Protein Vectors

Get 50% discount on the second and subsequent vectors when ordering two or more vectors with the same fluorescent protein.

Lentiviral Barcode Library

Lentiviral-based barcode library

Track clonal populations of cells over time or through different treatments using the lentiviral-based barcode library.

Support

Request a Catalog
Join our Mailing List
Contact us

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!