Transmigration Assays• Quantify cell transmigration with no manual cell counting
• Determine leukocyte/tumor endothelium interactions
• Highly sensitive results on a fluorescence plate reader
Transmigration describes the migration of cells (usually leukocytes or tumor cells) through the vascular endothelium toward a chemoattractant. The initial arrest and attachment of tumor cells to vascular endothelium precedes their extravasation from the blood stream and is a crucial step in the tumor metastatic cascade. Tumor cell extravasation is equivalent, in many respects, to the entry of leukocytes into inflammatory tissue. Leukocyte extravasation consists of multiple, consecutive processes including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration.
The leukocyte adhesion / transmigration cascade.
The CytoSelect Transmigration Assays provide a robust system for the quantitative determination of transmigrations and interactions between endothelium and leukocytes/cancer cells. They are Boyden chamber-based assays in which the cells transmigrate from the upper chamber (membrane inserts with 3 or 8 um pore size, see table below) through an endothelial monolayer into the lower chamber.
Migratory cells may be quantified on a fluorescence plate reader.
|CytoSelect Leukocyte Transmigration Assay Kit||CBA-212-CB||24 assays||651 €||DETAILS||Add to Cart|
|CytoSelect Tumor Transendothelial Migration Assay Kit||CBA-216-CB||24 assays||651 €||DETAILS||Add to Cart|