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Home -> Cell Biology -> Cell Migration -> Haptotaxis Assays

Haptotaxis Assays

• Quantify haptotaxis with no manual cell counting
• Measure haptotaxis in less than 6 hours with most cell types
• Collagen I- or fibronectin-coated membrane inserts
• Colorimetric or fluorometric detection

Cell haptotaxis describes cell migration toward or along a gradient of chemoattractants or adhesion sites in the extracellular matrix.
The CytoSelect Haptotaxis Assays are optimized for determining the migratory properties of cells. They utilize polycarbonate membrane inserts, 8 um pore size, ideal for epithelial cells and fibroblasts.

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The bottom side of the Boyden chamber insert is coated with collagen I or fibronectin. The membrane serves as a barrier to distinguish between migratory and non-migratory cells.
Migratory cells are able to extend protrusions towards the gradient of extracellular matrix density (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. Finally, the non-migrating cells are removed and the migratory cells are stained and quantified.

Principle of the Haptotaxis Assay, Colorimetric
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CytoSelect 24-Well Cell Haptotaxis Assay.
MDA-231 cells were seeded at 150,000 cells/well and allowed to migrate toward FBS for 4 hours. Migratory cells were stained according to the assay protocol.
Related Links

Cell Migration Assays
Cell Invasion Assays

 

 

Description Cat# Size Price    
CytoSelect 24-well Cell Migration Haptotaxis Assay (Collagen Coated) (8 um), Colorimetric CBA-100-COL-CB 12 assays 498 € DETAILS   Add to Cart 
CytoSelect 24-well Cell Migration Haptotaxis Assay (Collagen Coated) (8 um), Fluorometric CBA-101-COL-CB 12 assays 517 € DETAILS   Add to Cart 
CytoSelect 24-well Cell Migration Haptotaxis Assay (Fibronectin Coated) (8 um), Colorimetric CBA-100-FN-CB 12 assays 498 € DETAILS   Add to Cart 
CytoSelect 24-well Cell Migration Haptotaxis Assay (Fibronectin Coated) (8 um), Fluorometric CBA-101-FN-CB 12 assays 517 € DETAILS   Add to Cart 

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