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Green Fluorescent Protein

Cyto-Tracers™: Live-cell tracing for dynamic in vitro and in vivo studies of subcellular activities
Accurately mark organelles and follow their dynamics with GFP/RFP-fusion tracer proteins expressed from integrated lentivectors

• Stable lentivector-based system for cell tracing
• Ideal for co-localization studies with other proteins
• Monitor protein trafficking in real-time
• Constructs for organelles, vesicles and compartments
• Bright and photostable GFP or RFP is non-disruptive to cells

Molecular trafficking is a dynamic process in eukaryotic cells. The Cyto-Tracers™ provide the ability to light up cell compartments for monitoring movement and localization of organelles and tracing endocytosis and exocytosis.
Our partner SBI has created a line of lentivector-based Cyto-Tracers™ that utilize GFP- or RFP-fusion proteins to mark cellular compartments, organelles, vesicles and structures to enable long-term and in-depth experimentation. The Cyto-Tracers can be used in transfections as well as packaged into virus to create stable GFP/RFP tracer cell lines in primary cells, tumor cell lines and stem cells.

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MSCV or CMV Promoter
To ensure the expression of these constructs in a variety of cell types, constructs are available with two extensively tested promoters:
The Murine Stem Cell Virus promoter (MSCV) is useful for stem cells and hematopoietic cell types.
The Cytomegalovirus promoter (CMV)-driven constructs can be used for other, easier to infect cell types.

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References
Neumann, B et al. (2010) Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 464(7289):721-7.
Eng, CH et al. (2010) Ammonia derived from glutaminolysis is a diffusible regulator of autophagy. Sci Signal. 3(119):ra31.
Löw, K et al. (2010) A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury. Gene Ther. 17(5):577-91.
Merzlyak, EM et al. (2007) Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 4(7):555-7.
Shaner, NC et al. (2007) Advances in fluorescent protein technology. J Cell Sci. 120(Pt 24):4247-60.
Related Links

Vector for creating your own C-terminal fusions
Vector for creating your own N-terminal fusions
RFP Constructs


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Description Cat# Size Price    
Actin Cyto-Tracer Lenti Vector: pCT-Actin-GFP (pCMV, B-actin Tag), packaged into lentiviral particles CYTO110-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Actin Cyto-Tracer Lenti Vector: pCT-Actin-GFP (pCMV, B-actin Tag), Plasmid Version CYTO110-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Actin Cyto-Tracer Lenti Vector: pCT-Actin-GFP (pMSCV, B-actin Tag), packaged into lentiviral particles CYTO110-VB-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Actin Cyto-Tracer Lenti Vector: pCT-Actin-GFP (pMSCV, B-actin Tag), Plasmid Version CYTO110-PB-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Autophagasome Cyto-Tracer Lenti Vector: pCT-Autophago-GFP (pCMV, LC3 Tag), packaged into lentiviral particles CYTO109-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Autophagasome Cyto-Tracer Lenti Vector: pCT-Autophago-GFP (pCMV, LC3 Tag), Plasmid Version CYTO109-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Cytosol and Control Cyto-Tracer Lenti Vector: pCT-Cyto-GFpLuc (pMSCV, GFP+Luciferase both untagged), packaged into lentiviral particles CYTO121-VB-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Cytosol and Control Cyto-Tracer Lenti Vector: pCT-Cyto-GFpLuc (pMSCV, GFP+Luciferase both untagged), Plasmid Version CYTO121-PB-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Cytosol Cyto-Tracer Lenti Vector: pCT-Cyto-GFP (pCMV, untagged GFP), packaged into lentiviral particles CYTO118-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Cytosol Cyto-Tracer Lenti Vector: pCT-Cyto-GFP (pCMV, untagged GFP), Plasmid Version CYTO118-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Cytosol Cyto-Tracer Lenti Vector: pCT-Cyto-GFP (pMSVV, untagged GFP), packaged into lentiviral particles CYTO118-VB-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Cytosol Cyto-Tracer Lenti Vector: pCT-Cyto-GFP (pMSVV, untagged GFP), Plasmid Version CYTO118-PB-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Cytosol to Mitochondria Cyto-Tracer Lenti Vector: pCT-GFP-BAX (pCMV, BAX Tag), packaged into lentiviral particles CYTO115-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Cytosol to Mitochondria Cyto-Tracer Lenti Vector: pCT-GFP-BAX (pCMV, BAX Tag), Plasmid Version CYTO115-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Cytosol to Nucleus to Membrane Cyto-Tracer Lenti Vector: pCT- Catenin-GFP (pCMV, B-Catenin activation Tag), packaged into lentiviral particles CYTO116-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Cytosol to Nucleus to Membrane Cyto-Tracer Lenti Vector: pCT- Catenin-GFP (pCMV, B-Catenin activation Tag), Plasmid Version CYTO116-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Dendrite Membrane Cyto-Tracer Lenti Vector: pCT-CMV-PSD95-GFP (pCMV, PSD-95 Tag), packaged into lentiviral particles CYTO117-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Dendrite Membrane Cyto-Tracer Lenti Vector: pCT-CMV-PSD95-GFP (pCMV, PSD-95 Tag), Plasmid Version CYTO117-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Endoplasmatic Reticulum Cyto-Tracer Lenti Vector: pCT-ER-GFP (pCMV, ER target Tag), packaged into lentiviral particles CYTO103-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Endoplasmatic Reticulum Cyto-Tracer Lenti Vector: pCT-ER-GFP (pCMV, ER target Tag), Plasmid Version CYTO103-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Endosome Cyto-Tracer Lenti Vector: pCT-Endo-GFP (pCMV, RhoB Tag), packaged into lentiviral particles CYTO107-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Endosome Cyto-Tracer Lenti Vector: pCT-Endo-GFP (pCMV, RhoB Tag), Plasmid Version CYTO107-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
ER-Golgi Vesicles Cyto-Tracer Lenti Vector: pCT-Secretory-GFP (pCMV, Secretory consensus Tag), packaged into lentiviral particles CYTO105-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
ER-Golgi Vesicles Cyto-Tracer Lenti Vector: pCT-Secretory-GFP (pCMV, Secretory consensus Tag), Plasmid Version CYTO105-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Exosome Secretion Cyto-Tracer Lenti Vector: pCT-CD63-GFP (pCMV, CD63 Tetraspanin Tag), packaged into lentiviral particles CYTO120-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Exosome Secretion Cyto-Tracer Lenti Vector: pCT-CD63-GFP (pCMV, CD63 Tetraspanin Tag), Plasmid Version CYTO120-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Exosome Secretion Cyto-Tracer Lenti Vector: pCT-CD81-GFP (pCMV, CD81 Tetraspanin Tag), packaged into lentiviral particles CYTO124-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Exosome Secretion Cyto-Tracer Lenti Vector: pCT-CD81-GFP (pCMV, CD81 Tetraspanin Tag), Plasmid Version CYTO124-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Exosome Secretion Cyto-Tracer Lenti Vector: pCT-CD9-GFP (pCMV, CD9 Tetraspanin Tag), packaged into lentiviral particles CYTO122-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Exosome Secretion Cyto-Tracer Lenti Vector: pCT-CD9-GFP (pCMV, CD9 Tetraspanin Tag), Plasmid Version CYTO122-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Golgi Apparatus Cyto-Tracer Lenti Vector: pCT-Golgi-GFP (pCMV, Galactosyltrasferase Tag), packaged into lentiviral particles CYTO104-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Golgi Apparatus Cyto-Tracer Lenti Vector: pCT-Golgi-GFP (pCMV, Galactosyltrasferase Tag), Plasmid Version CYTO104-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Inner Leaflet of Membrane Cyto-Tracer Lenti Vector: pCT-InnerMem-GFP (pCMV, Farnesylation signal Tag), ), packaged into lentiviral particles CYTO101-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Inner Leaflet of Membrane Cyto-Tracer Lenti Vector: pCT-InnerMem-GFP (pCMV, Farnesylation signal Tag), Plasmid Version CYTO101-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Inner Leaflet of Membrane Cyto-Tracer Lenti Vector: pCT-InnerMem-GFP (pMSCV, Farnesylation signal Tag), packaged into lentiviral particles CYTO101-VB-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Inner Leaflet of Membrane Cyto-Tracer Lenti Vector: pCT-InnerMem-GFP (pMSCV, Farnesylation signal Tag), Plasmid Version CYTO101-PB-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Lysosome Cyto-Tracer Lenti Vector: pCT-Lyso-GFP (pCMV, Cathepsin B Tag), packaged into lentiviral particles CYTO106-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Lysosome Cyto-Tracer Lenti Vector: pCT-Lyso-GFP (pCMV, Cathepsin B Tag), Plasmid Version CYTO106-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Microtubules Cyto-Tracer Lenti Vector: pCT-MAP4-GFP (pCMV, MAP4 Tag), packaged into lentiviral particles CYTO112-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Microtubules Cyto-Tracer Lenti Vector: pCT-MAP4-GFP (pCMV, MAP4 Tag), Plasmid Version CYTO112-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Mitochondria Cyto-Tracer Lenti Vector: pCT-Mito-GFP (pCMV, COX8 Tag), packaged into lentiviral particles CYTO102-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Mitochondria Cyto-Tracer Lenti Vector: pCT-Mito-GFP (pCMV, COX8 Tag), Plasmid Version CYTO102-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Mitochondria Cyto-Tracer Lenti Vector: pCT-Mito-GFP (pMSCV, COX8 Tag), packaged into lentiviral particles CYTO102-VB-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Mitochondria Cyto-Tracer Lenti Vector: pCT-Mito-GFP (pMSCV, COX8 Tag), Plasmid Version CYTO102-PB-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Nucleus Cyto-Tracer Lenti Vector: pCT-H2B-GFP (pCMV, H2B Tag), packaged into lentiviral particles CYTO113-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Nucleus Cyto-Tracer Lenti Vector: pCT-H2B-GFP (pCMV, H2B Tag), Plasmid Version CYTO113-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Nucleus Cyto-Tracer Lenti Vector: pCT-H2B-GFP (pMSCV, H2B Tag), packaged into lentiviral particles CYTO113-VB-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Nucleus Cyto-Tracer Lenti Vector: pCT-H2B-GFP (pMSCV, H2B Tag), Plasmid Version CYTO113-PB-1-SBI 10 ug 698 € DETAILS   Add to Cart 
Peroxisome Cyto-Tracer Lenti Vector: pCT-Pero-GFP (pCMV, Peroxisome consensus Tag), packaged into lentiviral particles CYTO108-VA-1-GVO-SBI >2 x 10^6 IFUs 789 € DETAILS   Add to Cart 
Peroxisome Cyto-Tracer Lenti Vector: pCT-Pero-GFP (pCMV, Peroxisome consensus Tag), Plasmid Version CYTO108-PA-1-SBI 10 ug 698 € DETAILS   Add to Cart 
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