Mouse PreAdipocytes3T3 L1 mouse cells (deriving from Mouse Swiss Albino embryo) are fibroblast like cells, which differentiate into adipocytes when growing confluent.
Passage 9 3T3-L1 pre-adipocytes from our partner Zen-Bio are guaranteed to differentiate into adipocytes when using Zen-Bio´s protocol and media system (see link below)
Lipid accumulation in 3T3 L1 cells cultured in Zen-Bio media:
3T3-L1 preadipocytes were seeded in 24 well plates and differentiated per Zen-Bio´s protocol.
Cells were then fed Zen-Bio´s AM-1-L1, with fresh media being added every other day.
Phase contrast images were taken on day 7 (d7; A) and day 14 (d14; B) of differentiation using an Olympus IX60 microscope equipped with a STOP digital camera (20X magnification).
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