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Ready-To-Use Hydrogel

VitroGel™ 3D - Empower 3D cell research with simplicity and versatility
VitroGel™ 3D: Your Ready-to-use synthetic hydrogel system for 3D cell culture and beyond

• Ready-to-use - one simple mixing step with your cells/media at room temperature
• Fast gelation - 3D cell culture in 20 minutes
• Cells distribute homogeneously in the hydrogel
• Cell morphology and behavior grown in VitroGel 3D hydrogel system is similar to their natural state
• Transparent - compatible to different imaging systems for cell observation and downstream analysis
• Permeable - oxygen, nutrition etc. can easily move in/out the hydrogel system: great for drug discovery studies
• Cell harvesting - cells can be easily harvested from the hydrogel by using standard centrifuging methods
• Injectable - great for in vivo studies

- See demonstration videos link below -

Want to bridge in vitro and in vivo studies? But tired of inconsistency, undesired growth factors, cold solution temperature, complicated preparing steps, lack of natural ECM mimic, and the high expense of current 3D cell culture systems?
VitroGel™ 3D is an animal origin-free purely synthetic and cost-effective hydrogel system, which closely mimics the natural extracellular matrix (ECM) environment with a simple procedure step at room temperature.

VitroGel™ 3D: Simply the best for 3D Cell Culture

How does it work?

STAGE ONE (forming a soft hydrogel)
The hydrogel formation starts when mixing the VitroGel solution with cell culture medium. Hydrogel molecules interact with ions (such as Ca2+ or Na+) from the cell culture medium to become a matrix structure (hydrogel).

The process of hydrogel formation is slow when the ion concentration is low. At this stage, the hydrogel is soft and possesses a shear-thinning and rapid recovering mechanical property, which makes the hydrogel injectable for in vivo applications.

The slow hydrogel forming process and the injectable property of the soft hydrogel create a time window for easy hydrogel transfer from the mixing tube to the cell culture plate.


STAGE TWO (forming a solid hydrogel)
After transferring the soft hydrogel formation to a cell culture plate, adding additional cell culture medium on top of the hydrogel would increase the ion concentration, allowing more ions to penetrate into the hydrogel matrix to facilitate further hydrogel cross linking forming a solid hydrogel.

The solid hydrogel possesses higher mechanical strength than the soft hydrogel and can be broken into smaller gel pieces.

After the hydrogel solidifies, there is a clear gel-liquid phase separation, which allows medium change by removing and adding medium on top of the hydrogel.

In Vitro 3D Cell Models



In Vivo Studies


Our partner TheWell currently provides three versions of the hydrogel system:

VitroGel 3D – better for floating cell lines
VitroGel 3D-RGD (RGD peptide modified version) – better for adhesion cell lines
VitroGel 3D-RGD-PLUS – with high concentation of RGD peptide

Related Links

First Time User Notes
User Manual
Frequently Asked Questions
Use of VitroGel 3D: Demonstration Videos
Case Studies (includes list of cells and applications)



Description Cat# Size Price    
VitroGel 3D Ready-To-Use Hydrogel (sufficient for two 24-well plates) TWG001-TW 10 ml 321 € DETAILS   Add to Cart 
VitroGel 3D-RGD Ready-To-Use Hydrogel (sufficient for two 24-well plates) TWG002-TW 10 ml 386 € DETAILS   Add to Cart 
VitroGel RGD-PLUS TWG003-TW 10 ml 515 € DETAILS   Add to Cart 
VitroGel 3D Ready-To-Use Hydrogel (sufficient for 10 wells in 24-well plate) TWG001S-TW 2 ml 69 € DETAILS   Add to Cart 
VitroGel 3D-RGD Ready-To-Use Hydrogel (sufficient for 10 wells in 24-well plate) TWG002S-TW 2 ml 87 € DETAILS   Add to Cart 
VitroGel RGD-PLUS TWG003S-TW 2 ml 124 € DETAILS   Add to Cart 
VitroGel Dilution Solution Type 1 MS01-100-TW 100 ml 60 € DETAILS   Add to Cart 
VitroGel Dilution Solution Type 2 MS02-100-TW 100 ml 60 € DETAILS   Add to Cart 
VitroGel Cell Recovery Solution MS03-100-TW 100 ml 150 € DETAILS   Add to Cart 

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