The Cold Fusion technology developed by our partner SBI is a simple, rapid and highly efficient cloning method. It allows you to directly clone any PCR product(s) into any linearized expression vector, at any site. The PCR fragments are generated using a high-fidelity DNA polymerase like PRECISOR High-Fidelity DNA Polymerase, with primers that are designed to have at least 15 bases of homology with the ends of the linearized vector. The Cold Fusion Cloning Kit is so robust that even multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single step.
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*Expires 30 September 2015