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Dear Researcher,

The Cold Fusion technology developed by our partner SBI is a simple, rapid and highly efficient cloning method. It allows you to directly clone any PCR product(s) into any linearized expression vector, at any site. The PCR fragments are generated using a high-fidelity DNA polymerase like PRECISOR High-Fidelity DNA Polymerase, with primers that are designed to have at least 15 bases of homology with the ends of the linearized vector. The Cold Fusion Cloning Kit is so robust that even multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single step.

Cold Fusion Cloning Kit

  • Restriction enzyme-, phosphatase- and ligase-free system
  • Rapid one tube reaction - 5 min at RT, 10 min on ice
  • Cloning efficiencies >95%

PRECISOR High-Fidelity DNA Polymerase

  • Highest accuracy
  • High speed and processivity
  • High yields up to 30kb

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*Expires 30 September 2015

BioCat GmbH

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D-69120 Heidelberg
Tel:  +49 (0) 6221 7141516
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Email: info@biocat.com
Web: http://www.biocat.com