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Dear Researcher,

Our partner SBI has developed a non-viral technology that is a unique alternative to traditional retroviral-based reprogramming of cells.

Episomal iPSC Reprogramming System

  • Plasmid mixture with Oct4, Sox2, Klf4, L-Myc, Lin28, shRNA-p53, miR302/367
  • GFP marker included for monitoring delivery and loss
  • Simple protocol for reliable reprogramming
  • Efficient reprogramming of elderly fibroblast cells
  • Compatible with feeder or feeder-free conditions

The system consists of an optimized mixture of plasmids built in Epstein-Barr Nuclear Antigen-1 (EBNA-1) containing vectors. The presence of oriP / EBNA-1 leads to replication and partition of the plasmids into daughter cells during the cell cycle. The subsequent robust expression of the factors allows the generation of human iPS cells in a single transfection.

BioCat GmbH

Im Neuenheimer Feld 584
D-69120 Heidelberg
Tel:  +49 (0) 6221 7141516
Fax: +49 (0) 6221 7141529
Email: info@biocat.com
Web: http://www.biocat.com