Dear Researcher,

The recent discovery of the type II prokaryotic CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, originally discovered in the bacterium Streptococcus pyogenes, has provided a revolutionary tool for targeted genome engineering with simple elegance.

  • Specific genome cleavage using gRNAs
  • Easy gene knockouts
  • Efficient genome editing
  • All-in-one Cas9-gRNA cloning and expression vectors

Cas9 SmartNuclease System

The system uses CRISPR-associated endonuclease Cas9, that complexes with small RNAs as guides (gRNAs) to cleave DNA in a sequence-specific manner upstream of the protospacer adaptor motif (PAM) in any genomic location. The CRISPR-Cas9 system is comprised of separate guide RNAs known as the crRNA and tracrRNA. These two separate RNAs have been combined into a single RNA to enable site-specific mammalian genome cutting through the design of a short guide RNA.

Full overview of tools for gene editing

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