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Accelerate your CRISPR/Cas9 workflows with CRISPY Master Mix, a reagent that enables fast, sensitive, and accurate quantitation of genome editing success using qPCR. Leveraging a “snapback” primer and a DNA polymerase that lacks both 5' to 3' exonuclease and strand displacement activity, CRISPY assays can be used to measure the percentage of successful genome edits at the target site from purified genomic DNA or cells.

CRISPY Master Mix

for qPCR-based Gene Editing Quantitation

  • Quantitation of editing efficiency specifically at the target site
  • Fast workflows that are complete in < 1 hour with only 15 minutes of hands-on time
  • Simple, one-step workflows - no DNA purification required
  • Sensitive detection that can report success rates as low as 1%
  • True quantitation based on observed Ct instead of gel imaging
  • Low sample input requirements - works on as little as 0.5 ng DNA

CRISPR/Cas9 Genome Engineering Overview

BioCat GmbH

Im Neuenheimer Feld 584
D-69120 Heidelberg
Tel:  +49 (0) 6221 7141516
Fax: +49 (0) 6221 7141529
Email: info@biocat.com
Web: http://www.biocat.com