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Gibson Assembly cloning allows for insertion of DNA fragments into virtually any vector without the need for compatible restriction sites. Join any two fragments to generate seamless constructs in your desired vector.

10% OFF* Gibson Assembly Kits

Features of the Gibson Assembly Kits:

  HiFi 1-Step Kit Ultra Kit Site-Directed Mutagenesis Kit
Hands on time <5 min <6 min <40 min
Reaction time 60 min 80 min 4-6 hrs
No. of steps 1 2 3
Fragment size 500 bp - 32 kb 100 bp - 100 kb 500 bp - 5.5 kb
Efficiency >90% >95% 1 site: >95%, 5 sites: >55%
No. of fragments Up to 5 Up to 15 Up to 5

*Valid until 30 September 2018. Please mention Code GA10 when ordering.

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