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| Cat#: | MC101-EV |
| Quantity: | 100 ml |
| Price: | 65 € |
| Supplier: | Evrogen |
Photobleaching of fluorescent proteins in response to prolonged exposure to exciting radiation significantly affects their utility as in vivo labels. The only ways of minimizing photobleaching known to date were limited to reducing of the excitation light intensity or shortening the duration of illumination.
The new Evrogen DMEMgfp live cell visualization medium excludes the components that lead to oxidative reddening of green fluorescent proteins, the photochemical process that was shown to be one of the main sources of GFP photobleaching [Bogdanov et al., 2009, see link below]. Replacing the culture medium with DMEMgfp for the period of visualization results in up to a 9-fold increase of photostability of EGFP, a 3.3-fold increase of photostability of TagGFP2 and more than a 4-fold increase of photostability of activated forms of photoactivatable PA-GFP and PS-CFP2 (the effect varies for particular fusions and localizations).
Long-term fluorescent microscopy of EGFP in live HEK293T cells maintained in DMEM or DMEMgfp.
DMEMgfp medium is intended for imaging use only, and is not recommended for long-term cell culturing. Nevertheless, no negative effects on cell physiology were observed during the first week of cultivation in DMEMgfp.
Use: Replace your cell culture medium with DMEMgfp 20-30 min before starting imaging experiment. The replacement should be performed under sterile conditions.
Storage: Should be stored at +2 to - 8°C
