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New Monomeric Yellow fluorescent protein: TagYFP
• Bright yellow fluorescence
• Monomeric, successful performance in fusions
• High pH stability
TagYFP is a bright yellow mutant of wild-type GFP-like protein from jellyfish Aequorea macrodactyla. TagYFP possesses single excitation maximum at 508 nm, and emission maximum at 524 nm.
TagYFP Use
TagYFP is mainly intended for protein labeling. It can also be used for cell and organelle labeling and for tracking the promoter activity.
TagYFP´s successful performance in fusions has been demonstrated on the example of mitochondrial localization signal (N-fusion), cytoplasmic β-actin, and α-tubulin (C-fusions). The corresponding vectors were transiently transfected into mammalian cells. In all cases, an expected signal distribution was observed within 12 hrs after transfection.
Mitochondria labeling using TagYFP. Confocal image of a transiently transfected HeLa cell expressing mitochondria-targeted TagYFP.
TagYFP performance in fusion with β-actin. Confocal image of a transiently transfected HeLa cell expressing TagYFP fusion with β-actin
TagYFP performance in fusion with α-tubulin. Confocal image of a transiently transfected HeLa cell expressing TagYFP fusion with α-tubulin
Detailed Vector Description
Learn more about TagYFP Protein
Cell Lines stably transfected with fluorescent proteins
