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| Cat#: | STA-354-CB |
| Quantity: | 1 set |
| Price: | 220 € |
| Supplier: | Cell Biolabs |
The OxiSelect Comet Assay Control Cells are designed for use in a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage (under alkaline conditions). First, individual cells are mixed with molten agarose before application to the OxiSelect™ Comet Slide. These embedded cells are then treated with a lysis buffer and alkaline solution, which relaxes and denatures the DNA. Finally, the samples are electrophoresed in a horizontal chamber to separate intact DNA from damaged fragments. Following alkaline electrophoresis, the samples are dried, stained with a DNA dye, and visualized by epifluorescence microscopy. Under these conditions, the damaged DNA (containing cleavage and strand breaks) will migrate further than intact DNA and produce a “comet tail” shape. For a complete protocol, refer to the OxiSelect Comet Assay Kit insert (see link below).
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