|Quantity:||5 ug transfection-ready DNA|
Provided free of charge when purchasing GoClone 3'UTR reporter constructs and mentioning code EMPTY_3UTR
3’UTR Insert Sequencing Primers:
For sequence and annotation file see link below.
The empty 3’UTR vector contains no 3´UTR nor a control insert, only the luciferase gene (RenSP) and its constitutive promoter.
It may serve as normalization control, as positive transfection control (because the constitutive promoter is highly active in most cell types), or for creating your own 3´UTR constructs.
Example data for SwitchGear 3’UTR control vectors. Here 50ng of each vector was transfected with FuGene6 (Roche) into HT1080 cells. Absolute and relative activity will vary depending on cell line, experimental conditions, and transfection reagents. Values shown in log scale.
5 ug purified plasmid DNA
Quality >1.75 260/280 ratio, sequence verified
LightSwitch GoClones should be used with LightSwitch Assay Reagents for maximum sensitivity, brightness, and dynamic range