|Quantity:||5 ug transfection-ready DNA|
Random Genomic Sequence Control R02: Contains a non-conserved, non-genic, and non-repetitive human genomic fragment (chromosome coordinates chr2+: 122701379 – 122703948), cloned downstream of the RenSP luciferase reporter gene
Example data for SwitchGear 3’UTR control vectors. Here 50ng of each vector was transfected with FuGene6 (Roche) into HT1080 cells. Absolute and relative activity will vary depending on cell line, experimental conditions, and transfection reagents. Values shown in log scale.
5 ug purified plasmid DNA
Quality >1.75 260/280 ratio, sequence verified
LightSwitch GoClones should be used with LightSwitch Assay Reagents for maximum sensitivity, brightness, and dynamic range