LightSwitch GoClone Normalization Control Random Sequence 3'UTR Reporter Construct (Random Genomic DNA from chr2+) (R02_3UTR)

Cat#:	S890002-SW
Quantity:	5 ug transfection-ready DNA
Price:	250 €
Supplier:	SwitchGear Genomics

Data Sheet   User Manual  

Random Genomic Sequence Control R02: Contains a non-conserved, non-genic, and non-repetitive human genomic fragment (chromosome coordinates chr2+: 122701379 – 122703948), cloned downstream of the RenSP luciferase reporter gene



Example data for SwitchGear 3’UTR control vectors. Here 50ng of each vector was transfected with FuGene6 (Roche) into HT1080 cells. Absolute and relative activity will vary depending on cell line, experimental conditions, and transfection reagents. Values shown in log scale.


Product Specifications:

5 ug purified plasmid DNA
Concentration 30ng/ul
Quality >1.75 260/280 ratio, sequence verified


LightSwitch GoClones should be used with LightSwitch Assay Reagents for maximum sensitivity, brightness, and dynamic range

chromosome coordinates chr2+: 122701379 – 122703948
LightSwitch Go Clone 3 UTR Reporter Constructs and Controls
LightSwitch Luciferase Assay Reagents

Introduction to controls & normalization for 3’UTR GoClones
High-throughput Transfection Protocol for GoClone Assays
Protocol for K562 Suspension Cells