LightSwitch GoClone Negative Control Random Sequence Promoter Reporter Construct (Random Genomic DNA from chr16+) (R04_PROM)

Cat#:	S790004-SW
Quantity:	5 ug transfection-ready DNA
Price:	250 €
Supplier:	SwitchGear Genomics

Data Sheet   User Manual  

Random Genomic Sequence Control R04: Contains a 1 kb non-conserved, non-genic, and non-repetitive fragment from the human genome (chr16+:1645930-1646850) cloned upstream of the luciferase reporter.
Random Control Vectors produce slightly different signals than the empty vector and are considered optimal negative or background controls.



Expected data and analysis of housekeeping and random promoter control vectors: Random promoter constructs (R1, R2, R3, R4) serve as negative/background controls and establish baseline luciferase activity. The housekeeping promoter controls serve as positive controls and give relative luciferase activity.
Y-axis values shown in log scale.
Control vector activity may vary depending on cell line, treatment condition, and protocol changes.


Product Specifications:

5 ug purified plasmid DNA
Concentration 30ng/ul
Quality >1.75 260/280 ratio, sequence verified


LightSwitch GoClones should be used with LightSwitch Assay Reagents for maximum sensitivity, brightness, and dynamic range

Chromosome Coordinates chr16+:1645930-1646850
LightSwitch GoClone Promoter Reporter Constructs & Controls
LightSwitch Luciferase Assay Reagents

High-throughput Transfection Protocol for GoClone Assays
Protocol for K562 Suspension Cells