|Quantity:||5 ug transfection-ready DNA|
Random Genomic Sequence Control R04: Contains a 1 kb non-conserved, non-genic, and non-repetitive fragment from the human genome (chr16+:1645930-1646850) cloned upstream of the luciferase reporter.
Random Control Vectors produce slightly different signals than the empty vector and are considered optimal negative or background controls.
Expected data and analysis of housekeeping and random promoter control vectors: Random promoter constructs (R1, R2, R3, R4) serve as negative/background controls and establish baseline luciferase activity. The housekeeping promoter controls serve as positive controls and give relative luciferase activity.
Y-axis values shown in log scale.
Control vector activity may vary depending on cell line, treatment condition, and protocol changes.
5 ug purified plasmid DNA
Quality >1.75 260/280 ratio, sequence verified
LightSwitch GoClones should be used with LightSwitch Assay Reagents for maximum sensitivity, brightness, and dynamic range