LightSwitch GoClone Positive Control Promoter Construct for Housekeeping Gene GAPDH (GAPDH_PROM)

Cat#:	S721624-SW
Quantity:	5 ug transfection-ready DNA
Price:	250 €
Supplier:	SwitchGear Genomics

Data Sheet   User Manual  

This Housekeeping control construct contains the promoter for the common housekeeping gene GAPDH driving the RenSP luciferase reporter.
This construct serves as positive transfection control and may also serve as control for comparing signals between conditions if they are known to be unresponsive to the test condition.



Expected data and analysis of housekeeping and random promoter control vectors: Random promoter constructs (R1, R2, R3, R4) serve as negative/background controls and establish baseline luciferase activity. The housekeeping promoter controls serve as positive controls and give relative luciferase activity.
Y-axis values shown in log scale.
Control vector activity may vary depending on cell line, treatment condition, and protocol changes.


Product Specifications:

5 ug purified plasmid DNA
Concentration 30ng/ul
Quality >1.75 260/280 ratio, sequence verified


LightSwitch GoClones should be used with LightSwitch Assay Reagents for maximum sensitivity, brightness, and dynamic range

GAPDH Promoter Coordinates chr12+: 6513163 – 6514226
LightSwitch GoClone Promoter Reporter Constructs & Controls
LightSwitch Luciferase Assay Reagents

High-throughput Transfection Protocol for GoClone Assays
Protocol for K562 Suspension Cells