|Quantity:||5 ug transfection-ready DNA|
This Housekeeping control construct contains the promoter for the common housekeeping gene ACTB driving the RenSP luciferase reporter.
This construct serves as positive transfection control and may also serve as control for comparing signals between conditions if they are known to be unresponsive to the test condition.
Expected data and analysis of housekeeping and random promoter control vectors: Random promoter constructs (R1, R2, R3, R4) serve as negative/background controls and establish baseline luciferase activity. The housekeeping promoter controls serve as positive controls and give relative luciferase activity.
Y-axis values shown in log scale.
Control vector activity may vary depending on cell line, treatment condition, and protocol changes.
5 ug purified plasmid DNA
Quality >1.75 260/280 ratio, sequence verified
LightSwitch GoClones should be used with LightSwitch Assay Reagents for maximum sensitivity, brightness, and dynamic range