• Total Protein isolated from a wide variety of common and hard-to-obtain tissues
• Extensive quality control procedures ensure high quality protein
Zyagen offers Total Protein preparations from human, mouse, rat, dog, bovine, pig, rabbit, sheep, horse, guinea pig, hamster, chicken, cat and plant tissues.
- Western blotting
- Isoelectric focusing gels
- SDS-capillary electrophoresis
Tissues are freshly dissected and homogenized in modified RIPA buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis. The extracted protein is provided in RIPA buffer at a concentration of 5mg/ml and ready for immediate use.
The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
The Protein is stable for one year if stored properly at -70°C.