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| Cat#: | P-1026-050-EP |
| Quantity: | 50 rxns |
| Price: | 149 € |
| Supplier: | Epigentek |
• Fastest procedure available: Completion within 30 minutes
• Highest conversion rate available: >99.99% conversion of unmethylated cytosine into uracil
• Highest recovery: More than 90% of DNA loss prevented by unique DNA protection reagents
• As little as 0.2 ng DNA required
To perfect DNA bisulfite treatment for better DNA methylation analysis, Epigentek developed a next generation DNA bisulfite conversion technology and incorporated this technology into its new product, the BisulFlash DNA Modification Kit. The BisulFlash method enables the entire cytosine conversion reaction to be processed with 100% single stranded DNA, which leads to a significantly higher conversion efficiency and faster speed. Additionally, a unique protection reagent prevents over 90% of DNA loss, preserving important DNA recovery efficiency.
The BisulFlash DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation microarray, and pyrosequencing. Furthermore, based on its ability for
a complete cytosine conversion, it is specifically suitable for next generation methylation sequencing and pyrosequencing.
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Complete Cytosine Conversion
200 ng of genomic DNA isolated from 3 cancer cell lines was treated with the BisulFlash DNA Modification Kit. Next, the unconverted and converted DNA in each treated sample were determined using unconverted DNAspecific and converted DNA-specific primers (â-actin, 110 bp), respectively.
Graph: Real-time PCR; Gel image: End-point PCR. The treated DNA was completely converted, and no unconverted DNA was determined after 45 cycles.
