High-throughput DNA Modification
The fastest procedure available, which can be finished within 2 hours and 30 min
High throughput is achievable along with a 96-well filter format
Completely converts unmethylated cytosine into uracil - modified DNA > 99.98%
The lowest degradation of DNA in the modification process - more than 90% of DNA loss can be prevented
The lowest requirement of starting DNA for modification - only 1 ng
Simple, reliable, and consistent modification conditions
The Methylamp-96 DNA Modification Kit contains a complete set of essential components enabling efficient bisulfite-based DNA modification. The entire procedure can be completed within 2 hours and 30 minutes and produces far superior results than any other commercially available kits. The resulting modified DNA is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray analysis.
DNA is denatured by heating, which allows DNA denaturation and bisulfite modification to be carried out simultaneously. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer prevents the chemical and thermophilic degradation of DNA during bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to bind tightly to the column filter, thus residual sodium bisulfite and salts can be effectively removed and clean modified DNA eluted.