Fast and Efficient Bisulfite Modification
• The fastest procedure available on the market, which can be completed within 1 hour and 55 minutes
• Completely converts unmethylated cytosine into uracil - modified DNA > 99.98%
• The lowest degradation of DNA in the modification process - more than 90% of DNA loss can be prevented
• The lowest requirement of starting DNA for modification - only 50 pg or 20 cells
• Extremely simple, reliable, and consistent modification conditions
The Methylamp DNA Modification Kit is a complete set of essential components which enables DNA methylation analysis using Epigentek´s uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within a mere 1 hour and 55 minutes and produces far superior results than any other commercially available kits. The resulting modified DNA is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
The Methylamp DNA Modification Kit contains all reagents required for bisulfite conversion on a DNA sample. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer prevent the chemical and thermophilic degradation of DNA during bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to bind tightly to the column filter, thus residual sodium bisulfite and salts can be effectively removed and clean modified DNA eluted.
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A high-throughput version in 96-well format (Cat# P-1008-EP) as well as a fast DNA modification version using heating process (Cat# P-1010-EP) are also available.