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| Cat#: | CBA-150-5-CB |
| Quantity: | 5 x 96 rxns |
| Price: | 2205 € |
| Supplier: | Cell Biolabs |
Tumor sensitivity assays are intended to help predict the sensitivity of various tumors to chemotherapeutic
agents, with the intent of identifying the most effective treatment with the fewest side effects. With this
information, physicians can devise tailor made chemotherapy regiments and eliminate ineffective drugs, sparing
patients of unnecessary toxicity. Ideally, an in vitro tumor sensitivity assay must be reliable, sensitive, and
resemble the 3-D, in vivo environment (such as culturing in collagen gel or soft agar).
Traditionally, the soft agar colony formation assay is a common method to monitor anchorage-independent
growth, which measures proliferation in a semisolid culture media after 3-4 weeks by manual counting of
colonies.
Cell Biolabs´ CytoSelect™ 5 x 96-well In Vitro Tumor Sensitivity Assay does not involve subjective manual counting of colonies or require a 3-4 week incubation period. Instead cells are incubated only 6-8 days in a proprietary semisolid agar media before being solubilized, transferred and detected by the provided MTT Solution in a microtiter plate reader.
The CytoSelect™ 96-well In Vitro Tumor Sensitivity Assay provides a stringent, anchorage-independent model for chemosensitivity testing and potential anticancer drug screening.
Each kit provides sufficient quantities to perform 5 x 96 tests in 96-well microtiter plates.
Kit Components:
1. 10X CytoSelect™ Agar Matrix Solution (Part No. 114001): Five sterile tubes – 5 x 1.8 mL
2. CytoSelect™ Matrix Diluent (Part No. 114002): Five sterile bottlea – 5 x 4.0 mL
3. 5X DMEM Solution (Part No. 113002): Fifteen sterile tubes – 1.5 mL each
4. 1X Matrix Solubilization Buffer (Part No. 115001): Five sterile bottles – 5 x 20.0 mL
5. Detergent Solution (Part No. 113501): Five bottles – 10.0 mL each
6. MTT Solution (Part No. 113502): Five tubes – 1.0 mL each
