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| Cat#: | CBA-135-CB |
| Quantity: | 96 rxns |
| Price: | 585 € |
| Supplier: | Cell Biolabs |
Neoplastic transformation occurs via a series of genetic and epigenetic alterations that yield a cell population that is capable of proliferating independently of both external and internal signals that normally restrain growth. For example, transformed cells show reduced requirements for extracellular growth promoting factors, are not restricted by cell-cell contact, and are often immortal. Anchorage-independent growth is one
of the hallmarks of transformation, which is considered the most accurate and stringent in vitro assay for detecting malignant transformation of cells.
Traditionally, the soft agar colony formation assay is a common method to monitor anchorage-independent growth, which measures proliferation in a semisolid culture media after 3-4 weeks by manual counting of colonies.
Standard soft agar assays are usually performed in 100-mm or 60 mm dishes, where cells are allowed to grow inside a semisolid culture media for 3-4 weeks before sizable colonies appear. This method is quite cumbersome, time-consuming, and difficult when testing a large number of samples. Additionally, the manual counting of colonies is highly subjective; with varying colony sizes, it’s difficult to determine meaningful results.
Cell Biolabs CytoSelect™ Cell Transformation Assay does not involve subjective manual counting of colonies or require a 3-4 week incubation period. Instead cells are incubated only 6-8 days in a proprietary semisolid agar media before being solubilized, transferred and detected by the provided MTT Solution in a microtiter plate reader. Moreover, viable transformed cells can be easily recovered for further
culturing and testing, such as in protein and DNA array analysis and cancer vaccine development.
This format provides a quantitative, high-throughput method to accurately measure cell transformation, while the
short incubation time (6-8 days) makes it possible to assay cells transiently transfected with oncogenes or siRNA.
The CytoSelect™ Cell Transformation Kit (Cell Recovery Compatible) provides a robust system for measuring in vitro drug sensitivity, screening oncogenes and cell transformation inhibitors, and allows for transformed cell recovery.
Each kit provides sufficient quantities to perform 96 tests.
Advantages over Traditional Transformation Assays:
• Time Savings: 7 days vs. 21 days
• 96, 48, 24, 12, or 6-well Format
• No Manual Counting
• Colrimetric Detection
• More Accurate and Precise
• Easy-To-Follow Instructions
• Viable transformed cells can be recovered and used for other studies
Kit Components
1. 10X CytoSelect™ Agar Matrix Solution (Part No. 114001): One sterile tube – 1.8 mL
2. CytoSelect™ Matrix Diluent (Part No. 114002): One sterile bottle – 4.0 mL
3. 5X DMEM Solution (Part No. 113002): Three sterile tubes – 1.5 mL each
4. 10X Matrix Solubilization Solution (Part No. 114003): One sterile tube – 1.8 mL
5. Detergent Solution (Part No. 113501): One bottle – 10.0 mL
6. MTT Solution (Part No. 113502): One tube – 1.0 mL
