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| Cat#: | CBA-130-5-CB |
| Quantity: | 5 x 96 rxns |
| Price: | 1965 € |
| Supplier: | Cell Biolabs |
Neoplastic transformation occurs via a series of genetic and epigenetic alterations that yield a cell population that is capable of proliferating independently of both external and internal signals that normally restrain growth. For example, transformed cells show reduced requirements for extracellular growth promoting factors, are not restricted by cell-cell contact, and are often immortal. Anchorage-independent growth is one of the hallmarks of transformation, which is considered the most accurate and stringent in vitro assay for detecting malignant transformation of cells.
Traditionally, the soft agar colony formation assay is a common method to monitor anchorage-independent growth, which measures proliferation in a semisolid culture media after 3-4 weeks by manual counting of colonies. Standard soft agar assays are usually performed in 100-mm or 60 mm dishes, where cells are allowed to grow inside a semisolid culture media for 3-4 weeks before sizable colonies appear. This method is quite cumbersome, time-consuming, and difficult when testing a large number of samples. Additionally, the manual counting of colonies is highly subjective; with varying colony sizes, it’s difficult to determine meaningful results.
Cell Biolabs CytoSelect™ 5 x 96-well Cell Transformation Assay does not involve subjective manual counting of colonies or require a 3-4 week incubation period. Instead cells are incubated only 6-8 days in a semisolid agar media before being solubilized, lysed and detected by the patented CyQuant® GR Dye in a fluorescence plate reader. This format provides a quantitative, high-throughput method to accurately measure cell transformation. Additionally, the short incubation time (6-8 days) makes it possible to assay cells transiently transfected with oncogenes or siRNA.
The CytoSelect™ 5 x 96-well Cell Transformation Kit provides a robust system for screening oncogenes and cell transformation inhibitors. Each kit provides sufficient quantities to perform 5 x 96 tests in 96-well microtiter plates.
Advantages over Traditional Transformation Assays:
• Time Savings: 7 days vs. 21 days
• 96-well, High-Throughput Format
• No Manual Counting
• Fluorometric Detection
• More Accurate and Precise
• Easy-To-Follow Instructions
Protocol-at-a-Glance
1. Seed cells in soft agar media in a 96-well plate
2. Incubate for 6-8 days, allowing colonies to form
3. Solubilize and lyse the agar media/colony suspension
4. Transfer the lysate to a fluorometer plate and detect with CyQuant® Dye
Number of Tests: 5 x 96
Kit Components:
1) 10 x CytoSelect™ Agar Matrix Solution
2) 5X DMEM Solution
3) 10 x Agar Matrix Solubilization Solution
4) Detergent / Lysis Solution
5) CyQuant® GR Dye
New: A special version of the kit is available, which enables you to recover viable, transformed cells for further studies, see link below
Product Line Overview
Version with Viable Cell Recovery Capability
1 x 96-well Assay Kit Version
384-well Assay Kit Version
5 x 384-well Assay Kit Version
