|Sample Type:||Serum, Plasma|
|Sample Size:||10 ul|
|Range:||0.125 ng/ml - 8 ng/ml|
|Incubation Time:||3 hours|
Adiponectin circulates as multiple isoforms (1) and is widely recognized as a key protein involved in insulin regulation and metabolic syndrome. Of the different multimers, the high-molecular-weight (HMW) form of adiponectin activates adenosine monophosphate activated protein kinase phosphorylation most effectively (1).
HMW levels, or the ratio of HMW to total adiponectin, are more meaningful than the total adiponectin level for predicting insulin resistance and the development of metabolic syndrome (2).
Recently, an ELISA system was developed for the selective quantification of human adiponectin oligomers using protease pretreatment (3). For order info see link below.
The Mouse Adiponectin Total & HMW Kit reflects a similar system for simultaneous detection of Mouse HMW and Total adiponectin (4)
(1) Hada Y, et al. Biochem Biophys Res Commun 2007; 356: 487-93.
(2) Hara K, et al. Diabetes Care 2006; 29: 1357-62
(3) Ebinuma H, et al. Clin Chim Acta 2006; 372: 47-53
(4) Ebinuma H, et al. Clin Chim Acta 2009; 401: 181-3