Product Cart
| Cat#: | 30000-NB |
| Quantity: | 12 rxns |
| Price: | 250 € |
| Supplier: | Norgen |
• Purification of all sizes of circulating RNA incl. microRNA
• Versatile serum and plasma input volumes
• Convenient spin column format, processing time < 30 min
Free-circulating RNA in plasma and serum has the potential to provide biomarkers for certain cancers and disease states, and includes tumor-specific extracellular RNA in the blood. It is usually present as short fragments of less than 1000nt, and includes free-circulating microRNA.
The Norgen Plasma/Serum Circulating RNA Purification Kit provides an efficient method for the purification of all sizes of these fragmented free-circulating RNAs from human plasma or serum (1-5ml). The kit does not require extension tubes for the purification of free-circulating RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples, and the kit allows for the concentration of RNA that is present in low concentrations (1-100ng/ml circulating RNA in human plasma).
The purified plasma/serum free-circulating RNA is eluted in a solution that is compatible with reverse transcription qPCR, reverse transcription PCR, Northern Blot analysis, RNase protection and primer extension as well as expression array assays.
Isolation and detection of circulating RNA from serum and plasma.
The Plasma/Serum Circulating RNA Purification Kit was used to isolate circulating RNA from 5ml samples of serum and plasma that had been spiked with 1 x 10e6 HeLa cells. Five microlitres of the purified RNA were then used as template in qRT-PCR reactions to detect expression of the human 5S gene and the miR-21 gene. Both 5S rRNA and miR-21 could be detected from plasma (red and green triangles) and serum (red and green circles), indicating the high quality of the purified RNA. The black line corresponds to the non-template control.
Plasma/Serum Circulating NA Purification Kit (Slurry Format)
