PRECISOR High-Fidelity DNA Polymerase

Cat#: 1706-250-BL
Quantity: 250 Units
Price: 189 €
Supplier: BioCat

User Manual

• High-speed, high-fidelity thermostable DNA polymerase
• High processivity
• Amplification of templates up to 30kb
• Shorter PCR runs for longer templates
• Robust - minimal optimization required

PRECISOR High-Fidelity DNA Polymerase is a fast thermostable enzyme possessing 5’-3’ DNA polymerase and 3’-5’ proofreading exonuclease activities. PRECISOR High-Fidelity DNA Polymerase provides the highest fidelity of all commercially available thermostable DNA polymerases combined with enhanced processivity resulting in high speed DNA synthesis (15s/kb for templates of up to 5kb and 30s/kb for templates longer than 5kb).
PRECISOR High-Fidelity DNA Polymerase is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons.

Two buffers are provided with PRECISOR High-Fidelity DNA Polymerase:
5x HiFi Buffer for high-fidelity applications, and 5x GC Buffer for the amplification of GC-rich (GC content >65%) or difficult templates.


Applications

• Cloning techniques for which high fidelity is desirable
• Blunt-end cloning
• Amplification of difficult templates
• Site-directed mutagenesis


Fidelity of PRECISOR High-Fidelity DNA Polymerase

The fidelity of PRECISOR High-Fidelity DNA Polymerase was determined in direct comparison with the leading high-fidelity enyzme using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999) and found to be slightly higher.


Performance of PRECISOR High-Fidelity DNA Polymerase


Amplification of low amounts of a 1kb genomic DNA fragment using PRECISOR High-Fidelity DNA Polymerase results in high yields.
A 1kb fragment of the mouse 18S rRNA gene was amplified using 1.5 units of PRECISOR High-Fidelity DNA Polymerase and HiFi Buffer. Starting template amount was 6.25ng mouse genomic DNA (lane 1), followed by a series of 2-fold dilutions (lanes 2-8). PCR was performed in 50µl reaction mixtures containing 2mM MgCl₂. Extension time was 30s/kb. M: DNA Ladder; C: Non-template Control.



Only 20 PCR cycles are required for amplification of large genomic DNA fragments using PRECISOR High-Fidelity DNA Polymerase.
Indicated starting amounts of 10, 20, and 30kb-sized Lambda DNA fragments were amplified using 2 units of PRECISOR High-Fidelity DNA Polymerase and 20 PCR cycles. PCR was performed in 50µl reaction mixtures with GC Buffer and a final MgCl₂ concentration of 2 mM. Extension time was 30s/kb. M: DNA Ladder.


Contents
250 Units PRECISOR High-Fidelity DNA Polymerase
5x HiFi Buffer (contains 10mM Mg²⁺)
5x GC Buffer (contains 10mM Mg²⁺)
50mM MgCl₂ Solution

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