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BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516
Home -> Next Generation Sequencing

A set of tools facilitating sample preparation for next generation sequencing and making other cloning projects even more complete and efficient is offered.

Full-length-enriched cDNA Synthesis

The Mint-2 cDNA Synthesis Kit uses a PlugOligo-based cDNA synthesis method to enrich for full-length genes.

cDNA Normalization

Using the duplex-specific nuclease-based Trimmer cDNA Normalization Kit for the creation of equalized cDNA libraries enhances the gene discovery rate by decreasing the over-representation of the most abundant transcripts.

PCR Purification

With Norgen PCR Purification Kits you can efficiently purify PCR fragments from 100 bp to 15,000 bp for next generation sequencing

Bias-free Cloning

Transcription- and translation-free CloneSmart vectors and highly competent cells allow for the cloning of complete unbiased libraries enabling the generation of next generation sequencing data with no gaps.


DNA Sample Preparation Reagents for Next Generation Sequencing

Product End
Repair
Blunt
Ending
3'A-Tailing 3'G-Tailing Adaptor
Ligation
Adaptor
Fill-In
DNA Amp. Phosphor-
ylation
PCRTerminator Kit +             +
DNATerminator Kit +             +
T4 DNA Polymerase   +            
T4 DNA Polynucleotide Kinase   +           +
NxGen T4 DNA Ligase
600,000U/ml
120,000U/ml
        +      
Bst Adapter Fill-In Kit           +    
Bst DNA Polymerase 8U/µl
50U/ul
    +     +    
DNA Polymerase I Klenow Fragment, exo-     +          
PyroPhage 3173 DNA Polymerase, exo-       +     +  
EconoTaq DNA Polymerase     +       +  

20% OFF! Bisulfite Kits

Benefit from outstanding cytosine conversion rates and fast protocols using DNA Bisulfite Modification Kits

40% OFF! PathArrays

Apply pathway-specific DNA microarrays for growth factor-, apoptosis- or inflammation-related gene expression analysis.

New! Ultrasensitive Assays

Analyze cell proliferation and cytotoxicity using only small cell numbers.

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