A set of tools facilitating sample preparation for next generation sequencing and making other cloning projects even more complete and efficient is offered.
Full-length-enriched cDNA Synthesis
The Mint-2 cDNA Synthesis Kit uses a PlugOligo-based cDNA synthesis method to enrich for full-length genes.
cDNA Normalization
Using the duplex-specific nuclease-based Trimmer cDNA Normalization Kit for the creation of equalized cDNA libraries enhances the gene discovery rate by decreasing the over-representation of the most abundant transcripts.
PCR Purification
With Norgen PCR Purification Kits you can efficiently purify PCR fragments from 100 bp to 15,000 bp for next generation sequencing
Bias-free Cloning
Transcription- and translation-free CloneSmart vectors and highly competent cells allow for the cloning of complete unbiased libraries enabling the generation of next generation sequencing data with no gaps.
DNA Sample Preparation Reagents for Next Generation Sequencing
| Product | End Repair |
Blunt Ending |
3'A-Tailing | 3'G-Tailing | Adaptor Ligation |
Adaptor Fill-In |
DNA Amp. | Phosphor- ylation |
|---|---|---|---|---|---|---|---|---|
| PCRTerminator Kit | + | + | ||||||
| DNATerminator Kit | + | + | ||||||
| T4 DNA Polymerase | + | |||||||
| T4 DNA Polynucleotide Kinase | + | + | ||||||
| NxGen T4 DNA Ligase 600,000U/ml 120,000U/ml |
+ | |||||||
| Bst Adapter Fill-In Kit | + | |||||||
| Bst DNA Polymerase 8U/µl 50U/ul |
+ | + | ||||||
| DNA Polymerase I Klenow Fragment, exo- | + | |||||||
| PyroPhage 3173 DNA Polymerase, exo- | + | + | ||||||
| EconoTaq DNA Polymerase | + | + |

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