cDNA & ORF Clone Collections

Choose from the most comprehensive collections of ready-to-use cDNA & ORF clones including the Mammalian Gene Collection (MGC) of full-length cDNAs for human, mouse, and rat.

Minicircle Technology

Minicircles are episomal DNA vectors that are produced as circular expression cassettes devoid of any bacterial plasmid DNA backbone. Their smaller molecular size enables more efficient transfections and offers sustained expression over a period of weeks as compared to standard plasmid vectors that only work for a few days. Different Minicircle DNA Vectors are offered for cloning your gene of interest.

PiggyBac Transposon System

The PiggyBac Transposon System enables genes of interest with no size limit (10-100kb) to be easily mobilized into target genomes. The integration is reversible as the transposon can be removed from the genome, footprint-free.

Cold Fusion Cloning Technology

The Cold Fusion Cloning Kit allows you to directly clone any PCR product(s) to any linearized expression vector, at any site. It is an efficient and rapid one tube reaction, incubation at room temperature for 5 minutes is followed by 10 minutes on ice, resulting in a >95% positive cloning rate.

Cloning without Restriction Sites

Red/ET recombination is a powerful tool for DNA subcloning, e.g. from BACs, and DNA modification, overcoming the limitations of conventional methods with respect to fragment size and restriction sites.

Expressioneering technology allows for the seamless cloning of PCR-amplified DNA into specially designed expression vectors without the need for enzymes or purification steps.

Cloning Kits & Competent Cells

CloneSmart Cloning Kits are designed for general purpose cloning, for shotgun, cDNA and BAC library construction, for cloning A/T rich genomes and sequences, or for cloning problematic genomes and toxic genes.

Competent cells for different purposes including expression of toxic proteins are also offered.

Improved chemically competent or electrocompetent SoluBL21 cells for soluble mammalian protein expression.

PRECISOR High-Fidelity DNA Polymerase

PRECISOR High-Fidelity DNA Polymerase generates amplicons with the highest accuracy and speed, even from your most difficult templates.