Product CartStem Cell Differentiation Reporter System
Monitor stem cells during differentiation
• Monitor cell differentiation in real-time
• Track 5 lineages: neural, hematopoietic, myogenic, structural and signaling
• Lentivector-based reporters with cell-specific promoter
• Choice of fluorescent marker - copGFP or RFP
• Choice of selection marker - Zeo or TK
Cell differentiation can be tracked in real-time using the Differentiation Reporter Constructs. Cell- and stage-specific promoters drive the expression of a fluorescent protein and a selection marker in differentiated cells. Lentivector-based reporters for 5 different lineages including neurogenic, hematopoietic, myogenic, structural and signaling are offered. They can be used to develop new directed ifferentiation protocols and to study cell fate specification.
All Differentiation Reporter Constructs are available as lentivector plasmids or as pre-packaged lentiviral peparations. Positive and negative transduction control plasmids and pre-packaged viral particles are also available.
Neural (Astrocytes, Neurons, Microglia, Optic nerves)
Hematopoiesis (B-cells, T-cells, and Macrophages)
Myogenesis (Cardiac, Smooth & Skeletal Muscle)
Structural (Bone, Collagen, Skin)
Signaling (Islets, Beta-cells)
SBI Differentiation Reporter Vector.
Cell-specific promoters drive expression of the fluorescent protein and selection marker in the corresponding cell type. Different configurations with copGFP or RFP for sorting, and Zeocin resistance for positive selection or thymidin kinase for negative selection are available.
Stem cell differentiation tracking.
Different neural lineages can be tracked using specific differentiation reporter constructs. Cell-specific promoters: DCX (Doublecortin), GFAP (Glial Fibrillary Acidic Protein), GAD67 (GAD1, Glutamic Acid Decarboxylase 1), MAP2 (Microtubule-associated Protein 2).
Reference
Ravin, R. et al. (2008) Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 3:670-80.
Publication contains online Supplemental Materials with amazing Astrocyte differentiation movies with SBI´s GFAP Reporter.
Related Links
Pluripotency Reporter Vectors
Expression Constructs for Pluripotency Genes
Overview: Stem Cell Related Products
All Products for Transcription Factor Activity Determination
Contact us for the plasmid versions
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (plasmid) | SR501PA-1-SBI | 10 ug | 425 € | DETAILS |  |
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (pre-packaged lentiviral particles) | SR501VA-1-SBI | >2 x 10^6 IFUs | 540 € | DETAILS | |
| Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (plasmid) | SR500PA-1-SBI | 10 ug | 425 € | DETAILS | |
| Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (pre-packaged lentiviral particles) | SR500VA-1-SBI | >2 x 10^6 IFUs | 540 € | DETAILS | |
| Adipocyte Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse ALBP Promoter | SR10036VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Astrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human GFAP Promoter | SR10015VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Astrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse GFAP Promoter | SR10016VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Astrocyte Specific Stem Cell Differentiation Reporter Vector (pRedZeo, prepackaged lentiviral particles), Human GFAP Promoter | SR10051VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| B-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human B29 Promoter | SR1004VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| B-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse B29 Promoter | SR1005VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Beta cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Insulin Promoter | SR10028VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human MLC-2v Promoter | SR10011VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Tnnt2 Promoter | SR10012VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse ACTC Promoter | SR10010VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Cardiomyocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse Tnnt2 Promoter | SR10013VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Cardiomyocyte Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human ACTC Promoter | SR10049VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| CD8 T-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse CD8 Promoter | SR1006VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Chondrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse col2a1 Promoter | SR1001VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Epithelium Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Keratin 14 Promoter | SR10038VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Erythroid Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human HLA-Dra Promoter | SR1007VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Islet Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human NGN3 Promoter | SR10037VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Islet Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human PDX1 Promoter | SR10039VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Islet Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse PDX1 Promoter | SR10040VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Lymphocyte Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human LCK Promoter | SR10032VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Macrophage, Microglia Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse CD68 Promoter | SR1008VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Microglia Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human CD11b Promoter | SR10017VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Microglia Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse EMR1 Promoter | SR10018VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Microglia Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse iba-1 Promoter | SR10019VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Muller glia Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse CD44 Promoter | SR10020VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neural Stem Cell Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Nestin Promoter | SR10035VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neural Stem Cell Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Rat Nestin Promoter | SR10034VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human BM88 Promoter | SR10021VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse CAMK2a Promoter | SR10022VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse GAD67 Promoter | SR10023VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse Ta1 a-Tubulin Promoter | SR10025VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Rat NSE Promoter | SR10024VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Doublecortin (DCX) Promoter | SR10041VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human FABP7 Promoter | SR10048VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Neuron Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human MAP2 Promoter | SR10047VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oligodendrocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse MBP Promoter | SR10026VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Osteoblast Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human SPP1 Promoter | SR1002VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Osteoblast Specific Promoter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Osteocalcin Promoter | SR1003VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Pan T-cell Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human CD2 (ciii) Promoter | SR1009VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Photoreceptor Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Human Opsin Promoter | SR10027VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Skeletal Myocyte Specific Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse Myogenin Promoter | SR10050VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Smooth muscle myocyte Specific Promoter Reporter Vector / Stem Cell Differentiation Reporter Vector (Prepackaged lentiviral particles), Mouse SM22a Promoter | SR10014VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
