Product CartPluripotency Reporter Systems
Pluripotency Response Reporter Constructs
Monitor and quantitate Oct4 and Sox2 activity
Lentivector-based reporters with Oct4 or Sox2 response element
Dual reporting system with both GFP and luciferase
Optional constitutive EF1-Puro or -Neo selection cassette
The pluripotent state of your stem cells can be verified using Oct4 (CR4) or Sox2 (SRR2) conserved enhancer Pluripotency Response Reporter Constructs. These lentivector-based transcriptional reporters express copGFP and luciferase in response to Oct4 or Sox2 activity. They work for both human and mouse stem cells. Using Oct4 (CR4) or Sox2 (SRR2) Response Reporter Constructs, you can track pluripotency in living cells with GFP and quantitate activities of Oct4 or Sox2 with luciferase.
SBI Pluripotency Response Reporter Vector.
Oct4 (CR4) or Sox2 (SRR2) conserved enhancer elements are cloned upstream of a minimal CMV promoter driving copGFP and luciferase expression in pluripotent cells.
Pluripotency Promoter Reporter Constructs
Confirm and enrich for pluripotent stem cells
Lentivector-based reporters with Oct4 or Nanog promoter
Choice of fluorescent marker - copGFP or RFP
Choice of selection marker - Zeocin or thymidine kinase
Pluripotency can be tracked in living cells using the Pluripotency Promoter Reporter Constructs. These lentivector-based constructs express copGFP or RFP under control of a stem cell-specific Oct4 or Nanog promoter. Both human and mouse Oct4 and Nanog Promoter Reporter Constructs are available. They can be applied for live cell imaging, sorting and selection of pluripotent cells.
SBI Pluripotency Promoter Reporter Vector.
Oct4 or Nanog promoters drive expression of the fluorescent protein and selection marker in pluripotent cells. Different configurations with copGFP or RFP for sorting, and Zeocin resistance for positive selection or thymidin kinase for negative selection are available.
All Pluripotency Reporter Constructs are available as lentivector plasmids or as pre-packaged lentiviral preparations. Positive and negative transduction control plasmids and pre-packaged viral particles are also provided.
Verification of the pluripotent state of stem cells.
Oct4 (CR4) or Sox2 (SRR2) Response Reporters (left) and Oct4 or Nanog Promoter Reporters (right) were used to confirm the pluripotent state of human iPS cells and human embryonic stem cells, respectively.
Related Links
Contact us for the plasmid versions
Expression Constructs for Pluripotency Genes
Stem Cell Differentiation Reporter System
Overview: Stem Cell Related Products
All Products for Transcription Factor Activity Determination
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| Oct4 CR4 Pluripotency Response Reporter Vector without selection pGreenFire Oct4 CR4-mCMV (pre-packaged lentiviral particles) | SR20070-VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS |  |
| Oct4 CR4 Pluripotency Response Reporter Vector with Neo selection pGreenFire EF1- Neo Oct4 CR4-mCMV (pre-packaged lentiviral particles) | SR20070-VA-N-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oct4 CR4 Pluripotency Response Reporter Vector with Puro selection pGreenFire EF1- Puro Oct4 CR4-mCMV (pre-packaged lentiviral particles) | SR20070-VA-P-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Sox2 SRR2 Pluripotency Response Reporter Vector without selection pGreenFire Sox2 SRR2-mCMV (pre-packaged lentiviral particles) | SR20071-VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Sox2 SRR2 Pluripotency Response Reporter Vector with Neo selection pGreenFire EF1- Neo Sox2 SRR2-mCMV (pre-packaged lentiviral particles) | SR20071-VA-N-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Sox2 SRR2 Pluripotency Response Reporter Vector with Puro selection pGreenFire EF1- Puro Sox2 SRR2-mCMV (pre-packaged lentiviral particles) | SR20071-VA-P-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc) for cloning your own TREs or as negative transduction control (plasmid) | TR010PA-1-SBI | 10 ug | 489 € | DETAILS | |
| pGreenFire1-mCMV (pTRH1 mCMV dscGFP T2A Fluc) for cloning your own TREs or as negative transduction control (pre-packaged) | TR010VA-1-SBI | >2 x 10^6 IFUs | 630 € | DETAILS | |
| pGreenFire1-CMV as positive transduction control (plasmid) | TR011PA-1-SBI | 10 ug | 489 € | DETAILS | |
| pGreenFire-1-CMV as positive transduction control (pre-packaged) | TR011VA-1-SBI | >2 x 10^6 IFUs | 630 € | DETAILS | |
| Nanog (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Human Nanog) (pre-packaged lentiviral particles) | SR10030VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Nanog (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Human Nanog) (pre-packaged lentiviral particles) | SR10042VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Nanog (Human) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Human Nanog) (pre-packaged lentiviral particles) | SR10055VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Nanog (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Mouse Nanog) (pre-packaged lentiviral particles) | SR10031VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Nanog (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Mouse Nanog) (pre-packaged lentiviral particles) | SR10044VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Nanog (Mouse) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Mouse Nanog) (pre-packaged lentiviral particles) | SR10056VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oct4 (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Human Oct4) (pre-packaged lentiviral particles) | SR10033VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oct4 (Human) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Human Oct4) (pre-packaged lentiviral particles) | SR10043VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oct4 (Human) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Human Oct4) (pre-packaged lentiviral particles) | SR10053VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oct4 (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pGreenZeo Mouse Oct4) (pre-packaged lentiviral particles) | SR10029VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oct4 (Mouse) Pluripotency Promoter Reporter Vector with Zeo selection (pRedZeo Mouse Oct4) (pre-packaged lentiviral particles) | SR10045VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Oct4 (Mouse) Pluripotency Promoter Reporter Vector with negative TK selection (pRedTK Mouse Oct4) (pre-packaged lentiviral particles) | SR10054VA-1-SBI | >2 x 10^6 IFUs | 599 € | DETAILS | |
| Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (plasmid) | SR500PA-1-SBI | 10 ug | 425 € | DETAILS | |
| Negative Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-mCMV (pre-packaged lentiviral particles) | SR500VA-1-SBI | >2 x 10^6 IFUs | 540 € | DETAILS | |
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (plasmid) | SR501PA-1-SBI | 10 ug | 425 € | DETAILS | |
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pGreenZeo-CMV (pre-packaged lentiviral particles) | SR501VA-1-SBI | >2 x 10^6 IFUs | 540 € | DETAILS | |
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedZeo-CMV (plasmid) | SR10046PA-1-SBI | 10 ug | 425 € | DETAILS | |
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedZeo-CMV (pre-packaged lentiviral particles) | SR10046VA-1-SBI | >2 x 10^6 IFUs | 540 € | DETAILS | |
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedTK-CMV(plasmid) | SR10052PA-1-SBI | 10 ug | 425 € | DETAILS | |
| Positive Transduction Control Vector for Pluripotency / Stem Cell Differentiation Reporter Vector System: pRedTK-CMV (pre-packaged lentiviral particles) | SR10052VA-1-SBI | >2 x 10^6 IFUs | 540 € | DETAILS | |
