Mouse Induced Pluripotent Cells (Mouse iPS Cell Lines)

Verified Mouse iPS Cell Line together with matched source cells

• iPS Cells reprogrammed with Oct4, Sox2, cMyc and Klf4
• Differentiation enabled, develop directed differentiattion protocols
• Study the induced pluripotency process
• Investigate epigenetics markers
• Measure mRNA and miRNA signatures

A Mouse Induced Pluripotent Stem (iPS) Cell Line is offered with matched source fibroblasts. They enable you to directly compare the differentiated and the induced pluripotent state.

How the iPS Cell Lines are made
Mouse C57 Black 6 Embryonic Fibroblasts (MEFs) are transduced with high-titer iPSC virus to induce pluripotency. The result is high quality iPS cells with no fluorescent markers or drug selection required. The entire process takes up to 6 weeks.




Validation of Pluripotency


Stem cell markers SSEA-1 and Nanog were detected by immunocytochemistry. Alkaline phosphatase activity was confirmed using an AP Detection Kit. Teratoma assays were performed by subcutaneous injection of iPS cells into the dorsal flank of SCID mice. Teratomas were visualized after 4 weeks with hematoxylin and eosin staining.


Differentiation of Mouse iPS cells into mouse neural stem cells



Mouse iPS Cells were differentiated into neural stem cells which were shown to be capable of producing multiple neural lineages. The lineages were confirmed by using SBI´s Differentiation Lenti-reporters.


Kit Contents
2 x 10e5 Mouse iPS cells are delivered together with 2 x 10e5 Mouse Embryonic Fibroblast source cells, and 50 ml StemPure complete media.
StemPure Media for growing iPS cells or embryonic stem cells is also available separately in a larger quantity (500 ml).


References
Rossant, J. 2007. St:em cells: The magic brew. Nature 448, 260–262.
Takahashi, K. and Yamanaka, S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126: 663–676.
Takahashi K. et al. 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131: 861–72.
Park, IH et al. 2008. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 451:141–6.
Baker, Monya 2007. Adult cells reprogrammed to pluripotency, without tumors. Nature Reports Stem Cells. 2007–12–11.
Nakagawa, M et al. 2008. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nature Biotechnology. 26: 101–106.
Okita, K, et al. 2007. Generation of germline-competent induced pluripotent stem cells. Nature. 448:313–7
Yu, J. et al. 2007. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science 318: 1917–1920.


Custom iPS Cell Line Service
Provide cells from your human or mouse model system, and our partner SBI will reprogram the cells using lentivirus expressing Oct4, Sox2, Klf4, and c-myc.
After about 3 months you will receive your specific iPS Cell Line, certified to be pluripotent through marker staining. Please see link below to request a quotation.

Stem Cell Differentiation Reporter System
Ask for a Custom iPS Cell Line Service Quotation
Overview of Tools for Stem Cell Research

Description	Cat#	Size	Price
Mouse iPS Cell Line Set: Source Cells (Mouse Embryonic Fibroblasts) & certified Induced Pluripotent Cells	SC201A-1-SBI	2 x 10e5 cells each	895 €	DETAILS