Human Induced Pluripotent Cells (Human iPS Cell Lines)

Verified Human iPS Cell Line together with matched source cells

• iPS Cells reprogrammed with Oct4, Sox2, cMyc and Klf4
• Differentiation enabled, develop directed differentiation protocols
• Study the induced pluripotency process
• Investigate epigenetics markers
• Measure mRNA and miRNA signatures

A Human Induced Pluripotent Stem (iPS) Cell Line is offered with matched source fibroblasts. They enable you to directly compare the differentiated and the induced pluripotent state.

How the iPS Cell Lines are made
Human foreskin fibroblasts (HFFs) are transduced with high-titer iPSC virus to induce pluripotency. The result is high quality iPS cells with no fluorescent markers or drug selection required. The entire process takes up to 6 weeks.




Validation of Pluripotency
The pre-made iPS cell lines are ready for your research and are validated as follows:
Stem cell markers for SSEA-4 (see image below) and Nanog were determined by immunocytochemistry using primary antibodies for SSEA-4 and Nanog followed by fluorescent-labeled secondary Alexa594 antibodies. Detection of Alkaline Phosphatase activity was performed using an AP Detection Kit (see image below). Teratoma assays were performed by subcutaneous injection of 1x10e6 iPS cells into the dorsal flank of SCID mice. Teratomas were visualized after 4 weeks with hematoxylin and eosin staining.


Stem cell marker SSEA-4 was detected by immunocytochemistry.


Alkaline phosphatase activity was confirmed using an AP Detection Kit.


Kit Contents
2 x 10e5 human iPS Cells are delivered together with 50 ml StemPure complete media, as well as with 2 x 10e5 Human Neonatal Foreskin Fibroblast source cells, which are also available in larger quantitities (1 x 10e6) as separate product in 2 formats: From an individual source for generating your own induced pluripotent cells, or pooled for the use as feeder cells.
StemPure Media for growing iPS cells or embryonic stem cells is also available separately in a larger quantity (500 ml).


References
Rossant, J. 2007. St:em cells: The magic brew. Nature 448, 260–262.
Takahashi, K. and Yamanaka, S. 2006. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126: 663–676.
Takahashi K. et al. 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131: 861–72.
Park, IH et al. 2008. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 451:141–6.
Baker, Monya 2007. Adult cells reprogrammed to pluripotency, without tumors. Nature Reports Stem Cells. 2007–12–11.
Nakagawa, M et al. 2008. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nature Biotechnology. 26: 101–106.
Okita, K, et al. 2007. Generation of germline-competent induced pluripotent stem cells. Nature. 448:313–7
Yu, J. et al. 2007. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science 318: 1917–1920.


Custom iPS Cell Line Service
Provide cells from your human or mouse model system, and our partner SBI will reprogram the cells using lentivirus expressing Oct4, Sox2, Klf4, and c-myc.
After about 3 months you will receive your specific iPS Cell Line, certified to be pluripotent through marker staining. Please see link below to request a quotation.

Mouse iPS Cell Line
Ask for a Custom iPS Cell Line Service Quotation
Overview of Tools for Stem Cell Research

Description	Cat#	Size	Price
Human iPS Cell Line Set: Source Cells (Human Neonatal Foreskin Fibroblasts) & certified Induced Pluripotent Cells	SC101A-1-SBI	2 x 10e5 cells each	1095 €	DETAILS
Human Foreskin Fibroblasts (P1 passage, from individual genetic source) for iPS Cell Line Construction	PC501A-HFF-SBI	1 x 10e6 cells	475 €	DETAILS
Human Foreskin Fibroblasts (P1 passage, pooled) for use as feeder cells	PC502B-HFF-SBI	1 x 10e6 cells	355 €	DETAILS
Human Primary Dermal Fibroblasts (Post Natal)	aI001-f-DVB	500000 cells	459 €	DETAILS
Human Primary Dermal Fibroblasts (Pre Natal)	pI001-f-DVB	500000 cells	559 €	DETAILS