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Home -> Proteomics -> Transcription Factor Activity -> Gel Shift Assays (EMSA) from Signosis

Gel Shift Assays (EMSA) from Signosis

• No isotope required - sensitive HRP based chemiluminescent detection
• No probe preparation - biotin pre-labeled probes included in the kit
• Simple procedure – simple and straightforward assay

Transcription factors (TFs) are a group of cellular proteins that control gene expression. Once a TF is activated, it binds to its corresponding cis-element within promoter regions and mediates its target gene expression. Electrophoretic Mobility Shift Assay (EMSA) is a commonly used method for monitoring the activation of a TF in vitro.

Signosis´TF consensus sequences have been carefully selected based on well-known publications. They are used as biotinylated probe for detecting TF activity in human, mouse and rat nuclear extract samples. Each Signosis EMSA Kit is a ready to go kit including all reagents including the pre-labeled probe set as well as transfer membranes.

When the TF of interest is activated, it binds to the biotin-labeled probe, which is designed based on the consensus sequence of the TF binding. When the formed complex is subject to electrophoresis, it runs slower than the free probe, which can be easily distinguished. Two or more samples can be compared by the shift assays and quantitative differences can be determined by the density of the shifted band.

Image
EMSA assays of nuclear extracts of HeLa control and PMA-treated HeLa.
Lane1: Probe only; Lane: 2 HeLa; Lane 3: PMA-treated HeLa


Related Links

User Manual
Nuclear Extraction Kit


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Description Cat# Size Price    
EMSA Kit - Gel Shift Kit without probe GS-0000-SO 30 rxns 379 € DETAILS Order
AP1 EMSA Kit - Gel Shift Kit for AP1 protein (JUN/FOS) GS-0001-SO 30 rxns 389 € DETAILS Order
AP2 EMSA Kit - Gel Shift Kit for AP2 protein GS-0002-SO 30 rxns 389 € DETAILS Order
AP3 EMSA Kit - Gel Shift Kit for AP3 protein GS-0082-SO 30 rxns 389 € DETAILS Order
AP4 EMSA Kit - Gel Shift Kit for AP4 protein GS-0083-SO 30 rxns 389 € DETAILS Order
AR EMSA Kit - Gel Shift Kit for Androgen receptor GS-0003-SO 30 rxns 389 € DETAILS Order
ATF2 EMSA Kit - Gel Shift Kit for Activating transcription factor 2 GS-0004-SO 30 rxns 389 € DETAILS Order
Brn-3 EMSA Kit - Gel Shift Kit for POU domain, class 4, transcription factor 1 GS-0005-SO 30 rxns 389 € DETAILS Order
C/EBP EMSA Kit - Gel Shift Kit for CCAAT/enhancer binding protein (C/EBP),alpha GS-0006-SO 30 rxns 389 € DETAILS Order
CAR EMSA Kit - Gel Shift Kit for Nuclear receptor subfamily 1, group I, member 3 GS-0007-SO 30 rxns 389 € DETAILS Order
CBF EMSA Kit - Gel Shift Kit for CCAAT/enhancer binding protein (C/EBP), zeta GS-0008-SO 30 rxns 389 € DETAILS Order
CDP EMSA Kit - Gel Shift Kit for Cut-like homeobox 1, CCAAT displacement protein GS-0009-SO 30 rxns 389 € DETAILS Order
COUP-TF EMSA Kit - Gel Shift Kit for Nuclear receptor subfamily 2, group F, member 1 GS-0084-SO 30 rxns 389 € DETAILS Order
CREB EMSA Kit - Gel Shift Kit for cAMP responsive element binding protein 1 GS-0010-SO 30 rxns 389 € DETAILS Order
E2F-1 EMSA Kit - Gel Shift Kit for E2F transcription factor 1 GS-0011-SO 30 rxns 389 € DETAILS Order
EGR EMSA Kit - Gel Shift Kit for Early growth response GS-0012-SO 30 rxns 389 € DETAILS Order
ELK EMSA Kit - Gel Shift Kit for Member of ETS oncogene family GS-0013-SO 30 rxns 389 € DETAILS Order
ER EMSA Kit - Gel Shift Kit for Estrogen receptor GS-0014-SO 30 rxns 389 € DETAILS Order
Ets EMSA Kit - Gel Shift Kit for V-ets erythroblastosis virus E26 oncogene homolog 1 GS-0015-SO 30 rxns 389 € DETAILS Order
FAST-1(FOXH1) EMSA Kit - Gel Shift Kit for Forkhead box H1 GS-0016-SO 30 rxns 389 € DETAILS Order
FOXA1 EMSA Kit - Gel Shift Kit for Homeobox A1 GS-0063-SO 30 rxns 389 € DETAILS Order
FoxC1 EMSA Kit - Gel Shift Kit for Homeobox C1 GS-0062-SO 30 rxns 389 € DETAILS Order
FOXD3 EMSA Kit - Gel Shift Kit for Forkhead box D3 GS-0055-SO 30 rxns 389 € DETAILS Order
FOXG1 EMSA Kit - Gel Shift Kit for FOXbox G1 GS-0076-SO 30 rxns 389 € DETAILS Order
FOXO1 (FKHR) EMSA Kit - Gel Shift Kit for FOXbox O1 GS-0075-SO 30 rxns 389 € DETAILS Order
FREAC-2 (FOXF2) EMSA Kit - Gel Shift Kit for Forkhead-related activator 2 GS-0095-SO 30 rxns 389 € DETAILS Order
GAS/ISRE EMSA Kit - Gel Shift Kit for IFN-stimulated response element GS-0017-SO 30 rxns 389 € DETAILS Order
GATA EMSA Kit - Gel Shift Kit for GATA transcription factor GS-0018-SO 30 rxns 389 € DETAILS Order
Gfi-1 EMSA Kit - Gel Shift Kit for Growth factor independent 1 GS-0077-SO 30 rxns 389 € DETAILS Order
Gli-1 EMSA Kit - Gel Shift Kit for GLI zinc finger transcription factor GS-0072-SO 30 rxns 389 € DETAILS Order
GR/PR EMSA Kit - Gel Shift Kit for Glucocorticoid receptor GS-0019-SO 30 rxns 389 € DETAILS Order
HEN(NSCL-1) EMSA Kit - Gel Shift Kit for Helix-loop-helix protein 1 GS-0094-SO 30 rxns 389 € DETAILS Order
HIF1 EMSA Kit - Gel Shift Kit for Hypoxia inducible factor GS-0021-SO 30 rxns 389 € DETAILS Order
HNF-1 EMSA Kit - Gel Shift Kit for Hepatocyte Nuclear Factor 1 GS-0093-SO 30 rxns 389 € DETAILS Order
HNF-4 EMSA Kit - Gel Shift Kit for Hepatocyte nuclear factor 4 GS-0022-SO 30 rxns 389 € DETAILS Order
HOX4C EMSA Kit - Gel Shift Kit for HOX4C homobox GS-0085-SO 30 rxns 389 € DETAILS Order
HOXA-5 EMSA Kit - Gel Shift Kit for Homeobox A5 GS-0061-SO 30 rxns 389 € DETAILS Order
HSF EMSA Kit - Gel Shift Kit for Heat shock transcription factor 1 GS-0054-SO 30 rxns 389 € DETAILS Order
IRF EMSA Kit - Gel Shift Kit for Interferon regulatory factor GS-0023-SO 30 rxns 389 € DETAILS Order
KLF4 EMSA Kit - Gel Shift Kit for Kruppel-like factor 4 GS-0090-SO 30 rxns 389 € DETAILS Order
MEF2 EMSA Kit - Gel Shift Kit for Myocyte enhancer factor 2 GS-0024-SO 30 rxns 389 € DETAILS Order
Myb EMSA Kit - Gel Shift Kit for V-myb myeloblastosis viral oncogene homolog GS-0025-SO 30 rxns 389 € DETAILS Order
Myc-Max EMSA Kit - Gel Shift Kit for V-myc myelocytomatosis viral oncogene GS-0026-SO 30 rxns 389 € DETAILS Order
MyoD EMSA Kit - Gel Shift Kit for Myogenic differentiation 1 GS-0064-SO 30 rxns 389 € DETAILS Order
MZF EMSA Kit - Gel Shift Kit for Zinc finger type transcription factor MZF GS-0086-SO 30 rxns 389 € DETAILS Order
NF-1 EMSA Kit - Gel Shift Kit for Nuclear factor 1 GS-0027-SO 30 rxns 389 € DETAILS Order
NF-E2 EMSA Kit - Gel Shift Kit for Nuclear factor (erythroid-derived 2) GS-0029-SO 30 rxns 389 € DETAILS Order
NFAT EMSA Kit - Gel Shift Kit for Nuclear factor of activated T-cells GS-0028-SO 30 rxns 389 € DETAILS Order
NFkB EMSA Kit - Gel Shift Kit for Nuclear factor of kappa light polypeptide gene GS-0030-SO 30 rxns 389 € DETAILS Order
Nkx2-5 EMSA Kit - Gel Shift Kit for Homeobox protein Nkx-2.5 GS-0065-SO 30 rxns 389 € DETAILS Order

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