Product CartProtein A coupled Glass Beads for IgG Purification
• High binding capacity: 45 mg IgG/ml Resin - achieved by large binding surface
• Inert and incompressible glass-matrix
• Easy handling: load - wash – elute
• Slurry or prepacked FPLC columns - ready-to-use
• Variety of package sizes up to bulk (> 5 L)
• Easy Sanitization
TRISOPOR-Protein A consists of recombinant Protein A containing IgG-binding regions (S. aureus) covalently linked to porous glass beads. TRISOPOR is an inert glass matrix devoid of shrinking or swelling. Nanotechnology is employed to create bead pores with a large binding surface leading to the extremely high binding capacity of 45 mg IgG/ml matrix.
TRISOPOR-Protein A can be used for the purification of monoclonal as well as polyclonal antibodies of IgG type from serum, ascites or cell culture.
It is applicable in columns or batch procedures. TRISOPOR-Protein A is provided as a 50% slurry. Prepacked FPLC columns compatible with all common FPLC systems such as ÄKTA, BioCAD and BioSepra are also available.
IgG purification using a prepacked TRISOPOR-Protein A column.
A prepacked TRISOPOR-Protein A column (1 ml) was loaded with 2 ml of human serum diluted 1:1 with 20 mM sodium phosphate buffer pH 7.0 at a flow rate of 0.25 ml/min. After washing, IgG was eluted with 0.2 M Tris/Glycine pH 2.6 at a flow rate of 1.0 ml/min. 0.5 ml fractions were collected and analyzed by agarose gel electrophoresis.
WHS: Whole human serum. SA: Serum albumin. a: a-Globulins. b: b-Globulins. IgG: Immunoglobulin G.
An RSF (regulatory support file) is available after signing a confidentiality agreement, as some of the information is proprietary.
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User Manual Slurry
User Manual Columns
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-0001-VB | 1 ml | 100 € | DETAILS |  |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-0005-VB | 5 ml | 260 € | DETAILS | |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-0025-VB | 25 ml | 830 € | DETAILS | |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-0100-VB | 100 ml | 2340 € | DETAILS | |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-0250-VB | 250 ml | 3200 € | DETAILS | |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-0500-VB | 500 ml | 4200 € | DETAILS | |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-1000-VB | 1000 ml | 6500 € | DETAILS | |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-2000-VB | 2000 ml | 12000 € | DETAILS | |
| Trisopor-Protein A Glass Beads (50% Slurry Format) | 51908-5000-VB | 5000 ml | 29000 € | DETAILS | |
| Trisopor-Protein A Glass Beads (Prepacked Column Format, Column Size: 8mm ID x 100mm L) | 51900-0050-VB | Column Volume 5 ml | 720 € | DETAILS | |
| Trisopor-Protein A Glass Beads (Prepacked Column Format, Column Size: 8mm ID x 20mm L) | 51900-0010-VB | Column Volume 1 ml | 195 € | DETAILS | |
| Trisopor-Protein A Glass Beads (Prepacked Column Format, Column Size: 8mm ID x 50mm L) | 51900-0025-VB | Column Volume 2.5 ml | 420 € | DETAILS | |
