Maximum Efficiency DNA Transfection for Primary Neurons

NeuroFECT Transfection Reagent

• Ultra High Efficiency for Transfection into Primary Neurons
• Highest transfection efficiency for primary neurons
• Low cytotoxicity
• Simple and straightforward protocol

NeuroFECT Transfection Reagent is a novel biodegradable cationic polymer created specifically for optimal transfection of primary neuronal cells.

For references featuring the use of NeuroFECT Transfection Reagent, see Citation Search


Transfection of rat cortical neurons.
See also pdf link below.


Transfection of primary rat hippocampus cells.
Primary rat hippocampal cells were transfected with 0.5 µg and 1.0 µg of GFP expression plasmid using NeuroFECT Transfection Reagent. Forty-eight hours post-transfection, the cells were assayed for GFP expression by FACS analysis (see also pdf link below).

How does it work ?
During transfection, the polymer/DNA complexes (polyplexes) are endocytosed into the cells, where the polymer is biodegraded into small non-toxic molecules. The ability of NeuroFECT Transfection Reagent to biodegrade in vivo means there is essentially no cytotoxicity and therefore delivery of macromolecules into cells is maximized.

The reagent is available in two sizes.

NeuroFECT Transfection Reagent is shipped at room temperature. For maximum stability, store reagent at 4°C upon receipt. If stored properly, the hydrated NeuroFECT Transfection Reagentis stable for 6 months.

Detailed Data for Rat Cortical Neurons
Detailed Data for Rat Hippocampal Cells

Description	Cat#	Size	Price
NeuroFECT Transfection Reagent (0.75 ml)	T800075-GL	300 rxns (1 ug DNA/transfection)	410 €	DETAILS
NeuroFECT Transfection Reagent (5 x 0.75 ml)	T800750-GL	1500 rxns (1 ug DNA/transfection)	1649 €	DETAILS