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Molecular Beacon Synthesis and Purification Service

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Molecular Beacons are dual-labeled FRET probes incorporating a Black Hole Quencher dye and a fluorophore reporter molecule. They differ from TaqMan probes, due to the incorporation of a short (~5 bp) complementary sequence of bases at the 3 and 5 ends. These complementary sequences hybridize to form a stem structure which consequently raises melting temperature of the probe and allows for the discrimination of sequences differing by single nucleotides.

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Molecular Beacon Probe
Dual-labeled hairpin probe with sequence-specific loop, used with a pair of forward and reverse primers.

Multiplex assays using Molecular Beacons are now possible that can detect several targets simultaneously using multiple spectrally resolved fluorescent probes, see Multiplexing Recommendations Table (link below).

A large selection of fluorophores and dark quenchers to help you optimize the performance of your multiplex assays are available.


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Multiplexing Recommendations Table


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Description Cat# Size Price    
Molecular Beacon: 3' Pulsar 650 (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) MBO-B2P6-1-BS 1 umol Scale (provides > 30 nmol pure probe) 830 € DETAILS Order
Molecular Beacon: 3' Pulsar 650 (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) MBO-B2P6-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 600 € DETAILS Order
Molecular Beacon: 3' Pulsar 650 (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) MBO-B2P6-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 560 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) MBO-CGB1-1-BS 1 umol Scale (provides > 30 nmol pure probe) 645 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) MBO-CGB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 485 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) MBO-CGB1-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 450 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 645 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-1-BS 1 umol (provides > 30 nmol pure probe) 645 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 485 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-2-BS 200 nmol (provides > 10 nmol pure probe) 485 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 450 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' DABCYL (Black Hole Quencher) MBO-CGD-5-BS 50 nmol (provides > 2 nmol pure probe) 450 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) MBO-COB1-1-BS 1 umol Scale (provides > 30 nmol pure probe) 645 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) MBO-COB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 485 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) MBO-COB1-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 450 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 645 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-1-BS 1 umol (provides > 30 nmol pure probe) 645 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 485 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-2-BS 200 nmol (provides > 10 nmol pure probe) 485 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 450 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Orange 560 Fluorophore Reporter (Emission 559 nm) 3' DABCYL (Black Hole Quencher) MBO-COD-5-BS 50 nmol (provides > 2 nmol pure probe) 450 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 590 (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C590B2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 635 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 590 (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C590B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 475 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 590 (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C590B2-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 440 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) MBO-CAB2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 680 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) MBO-CAB2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 515 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) MBO-CAB2-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 475 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 680 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-1-BS 1 umol (provides > 30 nmol pure probe) 680 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 515 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-2-BS 200 nmol (provides > 10 nmol pure probe) 515 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-5-BS 50 nmol (provides > 2 nmol pure probe) 475 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-5-BS 50 nmol (provides > 2 nmol pure probe) 475 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 610 Fluorophore Reporter (Emission 610 nm) 3' DABCYL (Black Hole Quencher) MBO-CAD-5-BS 50 nmol (provides > 2 nmol pure probe) 475 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 635 (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C635B2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 680 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 635 (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C635B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 560 € DETAILS Order
Molecular Beacon: 5' CAL Fluor Red 635 (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) MBO-C635B2-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 475 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) MBO-FB1-1-BS 1 umol Scale (provides > 40 nmol pure probe) 620 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) MBO-FB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 470 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) MBO-FB1-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 435 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-1-BS 1 umol Scale (provides > 40 nmol pure probe) 620 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-1-BS 1 umol (provides > 40 nmol pure probe) 620 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 470 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-2-BS 200 nmol (provides > 10 nmol pure probe) 470 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 435 € DETAILS Order
Molecular Beacon: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' DABCYL (Black Hole Quencher) MBO-FD-5-BS 50 nmol (provides > 3 nmol pure probe) 435 € DETAILS Order
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) MBO-HB1-1-BS 1 umol Scale (provides > 30 nmol pure probe) 675 € DETAILS Order
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) MBO-HB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 510 € DETAILS Order
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) MBO-HB1-5-BS 50 nmol Scale (provides > 2 nmol pure probe) 470 € DETAILS Order
Molecular Beacon: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' DABCYL (Black Hole Quencher) MBO-HD-1-BS 1 umol Scale (provides > 30 nmol pure probe) 675 € DETAILS Order

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