Circulating DNA Isolation
Plasma/Serum Circulating DNA Isolation Kit
• Isolate inhibitor-free circulating DNA from plasma and serum
• Versatile input volumes
• Concentrate circulating DNA into small elution volumes
• Fast and convenient spin column protocol
• Aso available in slurry format
Free-circulating DNA in plasma and serum has the potential to provide biomarkers for certain cancers and disease states, and includes tumor-specific extracellular DNA fragments in the blood and fetal DNA in maternal blood. It is usually present as short fragments of less than 1000 bp.
The Norgen Plasma/Serum Circulating DNA Purification Kit (29900-NB) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 1 ml to 5 ml. Purification is based on using a silicon carbide resin as the separation matrix. The kit provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes.
DNA can be isolated from either fresh or frozen samples using this kit, and the kit also allows for the concentration of DNA that is present in low concentrations (1-100ng/ml circulating DNA in human plasma).
Typical yields of free-circulating DNA will vary depending on the input sample, as the amount of DNA present in plasma and serum will depend upon the health status of the individual and the level of nucleases present in the blood. Preparation time for multiple sample is less than 35 minutes. The purified plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
|Plasma/Serum Circulating DNA Isolation Maxi Kit slurry format||51300-NB||10 preps||188 €||DETAILS|
|Plasma/Serum Circulating DNA Isolation Midi Kit slurry format||51200-NB||20 preps||188 €||DETAILS|
|Plasma/Serum Circulating DNA Isolation Mini Kit slurry format||50600-NB||50 preps||154 €||DETAILS|