DNA Methylation Kits
It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other epigenetic diseases. Aberrant DNA methylation is mainly found in 5´-CpG-3´dinucleotides within promoters or in the first exon of genes, which is an important pathway for the repression of gene transcription in diseased cells.
There have been many methods for the detection of DNA methylation. All of these methods require a bisulfite-based DNA modification before starting methylation assays such as methylation-specific PCR, sequencing, restriction analysis, and others. The bisulfite-based DNA modification is used to discriminate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
BisulFlash DNA Modification Kit
• Fastest procedure available: Completion within 30 minutes
• Highest conversion rate available: >99.99% conversion of unmethylated cytosine into uracil
• Highest recovery: More than 90% of DNA loss prevented by unique DNA protection reagents
• As little as 0.2 ng DNA required
To perfect DNA bisulfite treatment for better DNA methylation analysis, Epigentek developed a next generation DNA bisulfite conversion technology and incorporated this technology into its new product, the BisulFlash DNA Modification Kit. The BisulFlash method enables the entire cytosine conversion reaction to be processed with 100% single stranded DNA, which leads to a significantly higher conversion efficiency and faster speed. Additionally, a unique protection reagent prevents over 90% of DNA loss, preserving important DNA recovery efficiency.
The BisulFlash DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation microarray, and pyrosequencing. Furthermore, based on its ability for
a complete cytosine conversion, it is specifically suitable for next generation methylation sequencing and pyrosequencing.
Methylamp DNA Modification Product Line
• Procedure completion within <2hrs
• >99.9% conversion of unmethylated cytosine
• As little as 50pg DNA or 20 cells required
• DNA loss prevented by unique DNA protection reagents
Epigentek´s Methylamp DNA Modification Kits are designed for the fast and efficient conversion of unmethylated cytosine into uracil.
The following Methylamp DNA Modification Kits are offered:
Methylamp Whole Cell Bisulfite Modification Kit for DNA methylation analysis and DNA modification directly from cells or tissues.
Methylamp DNA Bisulfite Modification Kit for bisulfite conversion of a DNA sample.
Methylamp Combo Kit for efficient DNA isolation and modification.
Methylamp-96 DNA Bisulfite Modification Kit for bisulfite conversion of a DNA sample in 96-well format.
Methylamp One-Step DNA Bisulfite Modification Kit for simultaneous DNA denaturation and bisulfite modification.
The Methylamp Universal Methylated DNA Kit provides a methylation-positive control for methylation studies. It contains enzymatically methylated human genomic DNA and all components for DNA modification.
Product Comparison Chart - DNA Bisulfite Modification & Methylation
|BisulFlash DNA Bisulfite Modification Kit (30 Minute DNA Bisulfite Conversion Kit for DNA Methylation Analysis)||P-1026-050-EP||50 rxns||152 €||DETAILS|
|Methylamp Whole Cell Bisulfite Modification Kit||P-1016-40-EP||40 rxns||188 €||DETAILS|
|Methylamp Whole Cell Bisulfite Modification Kit||P-1016-80-EP||80 rxns||319 €||DETAILS|
|Methylamp DNA Bisulfite Modification Kit||P-1001-1-EP||40 rxns||148 €||DETAILS|
|Methylamp DNA Bisulfite Modification Kit||P-1001-2-EP||80 rxns||239 €||DETAILS|
|Methylamp Combo-Kit: DNA Bisulfite Modification Kit and FitAmp DNA Isolation Kit for Serum, Plasma, and Body Fluids||P-1002-40-EP||40 rxns||248 €||DETAILS|
|Methylamp-96 DNA Bisulfite Modification Kit||P-1008-1-EP||96 rxns||238 €||DETAILS|
|Methylamp-96 DNA Bisulfite Modification Kit||P-1008-2-EP||192 rxns||389 €||DETAILS|
|Methylamp One-Step DNA Bisulfite Modification Kit||P-1010-1-EP||40 rxns||148 €||DETAILS|
|Methylamp One-Step DNA Bisulfite Modification Kit||P-1010-2-EP||80 rxns||239 €||DETAILS|
|Methylamp Universal Methylated DNA Kit||P-1011-1-EP||5 ug methylated DNA, reagents for 10 rxns||268 €||DETAILS|
|Methylamp Universal Methylated DNA Kit||P-1011-2-EP||10 ug methylated DNA, reagents for 20 rxns||369 €||DETAILS|
|DNA Methylation Detection Kit||K5082100-BC||50 rxns||309 €||DETAILS|