Cold Fusion Fast Cloning System: PCR based, universal, suitable for multiple fragment cloning

Join the revolution in new cloning technologies: Experience the thrill of the chill-cloning in less than 20 minutes

• Clone any insert, at any site within any vector
• Restriction enzyme, phosphatase and ligase free system
• Powerful technology to join multiple fragments at once
• Rapid one tube reaction for 5 minutes at room temperature, then fusion on ice for 10 minutes
• Introduce or correct mutations at will in cDNAs
• Amenable to high throughput cloning applications

The Cold Fusion technology is a simple, rapid and highly efficient cloning kit. It allows you to directly clone any PCR product(s) to any linearized expression vector, at any site.

The PCR fragments can be generated by High Fidelity DNA Polymerases like BioCat´s PRECISOR Polymerase (see link below) or Taq DNA polymerase, with primers that are designed to have at least 15 bases of homology at their linear ends to where you want the product to fuse.
The linearized vector can be generated by PCR or restriction enzymes (single or double cut). One tube simple reaction format, 5 minute incubation at room temperature then 10 minutes on ice, your PCR product(s) rapidly and accurately fuse into the linearized vector in the desired orientation. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single step. The system is highly efficient, with more than 95% positive cloning rate.

How it works
The 5x Cold Fusion Master mix contains a proprietary blend of enzymes that prepare the ends of DNA fragments for sequence-directed alignment. Compatible DNA ends are then efficiently fused and produce vector clones with great accuracy.



Apply Cold Fusion technology for cloning a single DNA fragment



Apply Cold Fusion technology for cloning multiple DNA fragments in the order you choose in a 1 tube reaction

Related Links

PRECISOR Thermostable High-Fidelity DNA Polymerase

PDF-Downloads - Will open in new browser window

Cold Fusion Quick Protocol
Cold Fusion User Manual

 

Description	Cat#	Size	Price
Cold Fusion Cloning Kit	MC010A-1-SBI	10 rxns	300 €	DETAILS
Cold Fusion Cloning Kit	MC100A-1-SBI	20 rxns	484 €	DETAILS
Cold Fusion Cloning Kit	MC101A-1-SBI	50 rxns	1019 €	DETAILS