Product CartPluripotency Induction: microRNA based
Generate iPS cells with high efficiency using the microRNA-302bcad/367 cluster
• miR-302bcad/367 cluster
• Lentivector features GFP+Puro
• Pre-packaged lentivirus ready to transduce
• Minicircle vector co-expresses GFP
• Pre-made minicircle DNA ready to transfect
Induce either human or mouse somatic cells into the pluripotent state using a single microRNA cluster expression vector. The Human or Mouse microRNA302bcad/367 expression cassettes are available in Lentivector formats as well as non-integrating Minicircle DNA vector formats.
The microRNA-based reprogramming method has been published to be more than 1,000-fold more efficient that the standard technique based on Oct4/Sox2/Klf4/Myc reprogramming transcription factors. The microRNA-derived iPSCs display similar morphologies, pluripotent marker expression, and teratoma formation, and the mouse miR-iPSCs contribute to successful chimera germline transmission.
Bar graph figure adapted from Anokye-Danso F. et al., 2011.
Reference
Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, Tian Y, Zhang Y, Yang W, Gruber PJ, Epstein JA, Morrisey EE. Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency. Cell Stem Cell. 2011 Apr 8;8(4):376-88.
| Description | Cat# | Size | Price | ||
|---|---|---|---|---|---|
| Lentivector Human miR-302bcad/367 Reprogrammer with GFP and Puro Marker (plasmid) | TDH101PA-GP-SBI | 10 ug | 1409 € | DETAILS |
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| Lentivector Human miR-302bcad/367 Reprogrammer with GFP and Puro Marker (virus) | TDH101VA-GP-GVO-SBI | >2 x 10^6 IFUs | 769 € | DETAILS |
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| Lentivector Human miR-302bcad/367 Reprogrammer with GFP Marker (plasmid) | TDH101PA-G-SBI | 10 ug | 1409 € | DETAILS |
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| Lentivector Human miR-302bcad/367 Reprogrammer with GFP Marker (virus) | TDH101VA-G-GVO-SBI | >2 x 10^6 IFUs | 769 € | DETAILS |
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| Lentivector Mouse miR-302bcad/367 Reprogrammer with GFP and Puro Marker (plasmid) | TDM102PA-GP-SBI | 10 ug | 1409 € | DETAILS |
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| Lentivector Mouse miR-302bcad/367 Reprogrammer with GFP and Puro Marker (virus) | TDM102VA-GP-GVO-SBI | >2 x 10^6 IFUs | 769 € | DETAILS |
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| Lentivector Mouse miR-302bcad/367 Reprogrammer with GFP Marker (plasmid) | TDM102PA-G-SBI | 10 ug | 1409 € | DETAILS |
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| Lentivector Mouse miR-302bcad/367 Reprogrammer with GFP Marker (virus) | TDM102VA-G-GVO-SBI | >2 x 10^6 IFUs | 769 € | DETAILS |
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| Minicircle Human miR-302bcad/367 Reprogrammer (parental vector) | TDH101MN-G-SBI | 10 ug | DETAILS | ||
| Minicircle Human miR-302bcad/367 Reprogrammer (pre-made minicircles) | TDH101MC-G-SBI | 30 ug | DETAILS | ||
| Minicircle Mouse miR-302bcad/367 Reprogrammer (parental vector) | TDM102MN-G-SBI | 10 ug | DETAILS | ||
| Minicircle Mouse miR-302bcad/367 Reprogrammer (pre-made minicircles) | TDM102MC-G-SBI | 30 ug | DETAILS | ||
| ZYCY10P3S2T E. coli Minicircle Producer Strain (chemically competent cells, ready to transform) | MN900A-1-GVO-SBI | 5 vials | 512 € | DETAILS |
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