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Human Induced Pluripotent Cells (Human iPS Cell Lines)

Jump start your iPS cell research with pre-made, high quality iPS cell lines

• iPS cell lines made using Oct4, Sox2, c-Myc, and KLF4
• Study the induced pluripotency process
• Measure mRNA and microRNA signatures
• Identify novel epigenetic markers to study pattern formation
• Develop directed differentiation protocols


All iPS Cell lines are certified to be pluripotent through iPS Cell Marker staining

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Stem cell markers for SSEA4/1 and Nanog were determined by immunocytochemistry using primary antibodies for SSEA1 (Millipore), SSEA4 (Abcam) and Nanog (Abcam) followed by fluorescent-labeled secondary Alexa594 antibodies (Invitrogen). Detection of Alkaline Phosphatase activity was performed using the AP Detection kit (Millipore). Teratoma assays were performed by subcutaneous injection of 1x10^6 iPS cells into the dorsal flank of SCID mice. Teratomas were visualized after 4 weeks with hematoxylin and eosin staining.

Quality Source and Feeder Cells for Reliable Reprogramming

Human Epidermal Keratinocytes, neonatal cells only or complete kit:

• Highly pure, passage 1 neonatal epidermal keratinocytes optimized for iPSC generation
• Isolated from individual foreskin, single genetic background
• Human epidermal keratinocytes have been demonstrated to reprogram at least 100-fold more efficiently and at twice the speed of fibroblasts
• Keratinocytes are the only cell type of ectodermal origin—best source cells for Neural studies

Product formats

Neonatal Epidermal Keratinocyte Cells for iPSC generation (Cat#PC503hEKTN-C)

Human Epidermal Keratinocytes (Primary cells, Passage 1)
Note the "cobblestone-like" morphology
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Human foreskin fibroblasts, neonatal (Cat#PC501A-HFF)

• Highly pure neonatal foreskin fibroblasts optimized for iPSC generation
• Isolated from individual foreskin, single genetic background
• Measure mRNA and microRNA signatures
• Two times more cells/vial compared with competitors’ product

Highly pure neonatal foreskin fibroblasts for Feeder Cells (Cat#PC502B-HFF)

• Highly pure neonatal foreskin fibroblasts
• Pooled neonatal foreskin fibroblasts optimized as feeder cells

Human Foreskin Fibroblasts (Primary cells, Passage 1)
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Custom iPS Cell Line Service
Provide cells from your human or mouse model system, and our partner SBI will reprogram the cells using lentivirus expressing Oct4, Sox2, Klf4, and c-myc.
After about 3 months you will receive your specific iPS Cell Line, certified to be pluripotent through marker staining. Please see link below to request a quotation.


Related Links

StemPure Growth Medium
Mouse iPS Cell Line
Ask for a Custom iPS Cell Line Service Quotation
Overview of Tools for Stem Cell Research


 

Description Cat# Size Price    
Human Adipose Stem Cell-derived (feeder-free) iPS cells (hASC-iPSCs) SC402A-1-GVO-SBI 2 x 10e5 cells 1242 € DETAILS Order
Human Fibroblast-derived (feeder-free) iPSC Cell Line, includes 500 ml PSGro iPSC Growth Media (SC500M-1) SC102A-1-GVO-SBI 2 x 10e5 cells 1219 € DETAILS Order
Human Foreskin Fibroblasts (P1 passage, from individual genetic source) for iPS Cell Line Construction PC501A-HFF-SBI 1 x 10e6 cells 497 € DETAILS Order
Human Foreskin Fibroblasts (P1 passage, pooled) for use as feeder cells PC502B-HFF-SBI 1 x 10e6 cells 373 € DETAILS Order
Human Epidermal Keratinocytes (Cryopreserved) for iPS Cell Line (iPSC) Construction PC503hEKTN-C-SBI 1 x 10^6 cells 497 € DETAILS Order
Human Epidermal Keratinocytes for iPSC Construction, complete kit with keratinocytes, culture medium and neutralization solution PC503hEKTN-K-SBI 1 kit 926 € DETAILS Order

 


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