Cell Transformation Assay

CytoSelect™ 96-well and 384-well Cell Transformation Kits

Neoplastic transformation occurs via a series of genetic and epigenetic alterations that yield a cell population that is capable of proliferating independently of both external and internal signals that normally restrain growth. For example, transformed cells show reduced requirements for extracellular growth promoting factors, are not restricted by cell-cell contact, and are often immortal. Anchorage-independent growth is one of the hallmarks of transformation, which is considered the most accurate and stringent in vitro assay for detecting malignant transformation of cells.
Traditionally, the soft agar colony formation assay is a common method to monitor anchorage-independent growth, which measures proliferation in a semisolid culture media after 3-4 weeks by manual counting of colonies. Standard soft agar assays are usually performed in 100-mm or 60 mm dishes, where cells are allowed to grow inside a semisolid culture media for 3-4 weeks before sizable colonies appear. This method is quite cumbersome, time-consuming, and difficult when testing a large number of samples. Additionally, the manual counting of colonies is highly subjective; with varying colony sizes, it’s difficult to determine meaningful results.

Cell Biolabs CytoSelect™ Cell Transformation Assays do not involve subjective manual counting of colonies or require a 3-4 week incubation period. Instead cells are incubated only 6-8 days in a semisolid agar media before being solubilized, lysed and detected by the patented CyQuant® GR Dye in a fluorescence plate reader or by MTT in a colorimetric manner.
This format provides a quantitative, high-throughput method to accurately measure cell transformation. Additionally, the short incubation time (6-8 days) makes it possible to assay cells transiently transfected with oncogenes or siRNA.

The CytoSelect™ Cell Transformation Kits provide a robust system for screening oncogenes and cell transformation inhibitors. Each kit provides sufficient quantities to perform 96, 5 x 96, 384, or 5 x 384 tests in microtiter plates.
Special kit versions are available, which enable you to recover viable, transformed cells for further studies.

Advantages over Traditional Transformation Assays:

• Time Savings: 7 days vs. 21 days
• 96- or 384-well, High-Throughput Format
• No Manual Counting
• Fluorometric or Colorimetric Detection
• More Accurate and Precise
• Easy-To-Follow Instructions


Protocol-at-a-Glance

1. Seed cells in soft agar media in a 96-well or 384-well plate
2. Incubate for 6-8 days, allowing colonies to form
3. Solubilize and lyse the agar media/colony suspension
4. Transfer the lysate to a plate and detect with CyQuant® Dye or MTT


Number of Tests: 96, 5 x96, 384, or 5 x 384

Kit Components:

1) 10 x CytoSelect™ Agar Matrix Solution
2) Matrix Diluent
3) 5X DMEM Solution
4) 10 x Matrix Solubilization Solution*
5) Detergent/Lysis Solution
6) CyQuant® GR or MTT Dye

* In the CytoSelect 96-well In Vitro Tumor Sensitivity Assay
1 x Matrix Solubilization Solution is provided


For user manuals see individual products

Description	Cat#	Size	Price
CytoSelect 384-well Cell Transformation Assay (Soft Agar Colony Formation), Fluorometric detection	CBA-145-5-CB	5 x 384 rxns	3020 €	DETAILS
CytoSelect 384-well Cell Transformation Assay (Soft Agar Colony Formation), Fluorometric detection	CBA-145-CB	384 rxns	795 €	DETAILS
CytoSelect 96-well Cell Transformation Assay (Soft Agar Colony Formation) (Cell Recovery Compatible Version), Colorimetric detection	CBA-135-5-CB	5 x 96 rxns	2415 €	DETAILS
CytoSelect 96-well Cell Transformation Assay (Soft Agar Colony Formation) (Cell Recovery Compatible Version), Colorimetric detection	CBA-135-CB	96 rxns	610 €	DETAILS
CytoSelect 96-well Cell Transformation Assay (Soft Agar Colony Formation) (Cell Recovery Compatible Version), Fluorometric detection	CBA-140-5-CB	5 x 96 rxns	2520 €	DETAILS
CytoSelect 96-well Cell Transformation Assay (Soft Agar Colony Formation) (Cell Recovery Compatible Version), Fluorometric detection	CBA-140-CB	96 rxns	635 €	DETAILS
CytoSelect 96-well Cell Transformation Assay (Soft Agar Colony Formation), Fluorometric detection	CBA-130-5-CB	5 x 96 rxns	2055 €	DETAILS
CytoSelect 96-well Cell Transformation Assay (Soft Agar Colony Formation), Fluorometric detection	CBA-130-CB	96 rxns	535 €	DETAILS
CytoSelect 96-well In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation), Colorimetric detection	CBA-150-5-CB	5 x 96 rxns	2310 €	DETAILS
CytoSelect 96-well In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation), Colorimetric detection	CBA-150-CB	96 rxns	580 €	DETAILS