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Yellow Fluorescent Proteins (YFP s)

TagYFP Monomeric Yellow Fluorescent Protein

Bright yellow fluorescence
Monomeric, successful performance in fusions
High pH stability

TagYFP is a bright yellow mutant of wild-type GFP-like protein from jellyfish Aequorea macrodactyla. TagYFP possesses single excitation maximum at 508 nm, and emission maximum at 524 nm.

TagYFP Use
TagYFP is mainly intended for protein labeling. It can also be used for cell and organelle labeling and for tracking the promoter activity.
TagYFPs successful performance in fusions has been demonstrated on the example of mitochondrial localization signal (N-fusion), cytoplasmic β-actin, and α-tubulin (C-fusions). The corresponding vectors were transiently transfected into mammalian cells. In all cases, an expected signal distribution was observed within 12 hrs after transfection.

Image
Mitochondria labeling using TagYFP. Confocal image of a transiently transfected HeLa cell expressing mitochondria-targeted TagYFP.

Image
TagYFP performance in fusion with β-actin. Confocal image of a transiently transfected HeLa cell expressing TagYFP fusion with β-actin

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TagYFP performance in fusion with α-tubulin. Confocal image of a transiently transfected HeLa cell expressing TagYFP fusion with α-tubulin


TurboYFP Yellow Fluorescent Protein

True yellow fluorescence
Super bright signal
Super fast protein maturation
No cell toxicity

TurboYFP is an enhanced version of PhiYFP, modified yellow fluorescent protein from jellyfish Phialidium sp. Possessing super-bright yellow fluorescence with emission maximum at 538 nm, TurboYFP is ideally positioned between green and red fluorescent proteins allowing easy separation of these fluorescent tags by flow cytometry using common channels of detection and a single laser excitation line. Comparing to PhiYFP, TurboYFP matures faster in mammalian cells.

TurboYFP Use
TurboYFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and for tracking the promoter activity. Despite its dimeric structure, TurboYFP is still suitable for generation of fusions.
Both pTurboYFP-C and pTurboYFP-N vectors can be used to express TurboYFP alone in mammalian cells, however pTurboYFP-N vector is preferable for this application.

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TurboYFP use for in vivo labeling. Fluorescent microscopy of mammalian cells expressing TurboYFP alone (A), TurboYFP fusion with a mitochondrial targeting signal (B), TurboYFP fusion to cytoplasmic β-actin (C,D).


PhiYFP Yellow Fluorescent Protein

Super bright true-yellow fluorescence
Successful performance in fusions

PhiYFP and PhiYFP-m are the mutants of a natural yellow fluorescent protein from Phialidium sp. Mutagenesis resulted in a PhiYFP mutant that possesses very bright yellow fluorescence and demonstrates successful performance in N-terminal fusions. Another protein, PhiYFP-m, was optimized to generate fusions to its C-terminus.
Comparison of PhiYFP proteins with other available yellow fluorescent proteins reveals that they display the brightest true yellow fluorescence. The emission maximum of PhiYFP/PhiYFP-m is more than 10 nm longer than that of EYFP derived from A. victoria and can be more easily distinguished from green fluorescent proteins when used as the second color in multicolor applications. The emission wavelength of PhiYFP/PhiYFP-m is ideally positioned between those of green and red fluorescent proteins allowing easy separation of these fluorescent tags by flow cytometry using common channels of detection and a single laser excitation line.


Reference
Shagin et al., GFP-like proteins as ubiquitous Metazoan superfamily: evolution of functional features and structural complexity.
Mol. Biol. Evol. 2004, 21(5):841-850.

Related Links

pTagYFP-C Vector
pTagYFP-N Vector
pTurboYFP-C Vector
pTurboYFP-N Vector
pTurboYFP cDNA Vector
Cell Lines stably transfected with fluorescent proteins
Destabilized TurboYFP Vector
Promoterless TurboYFP Reporter Vector
Destabilized promoterless TurboYFP Vector
pPhiYFP-C Vector
pPhiYFP-N Vector


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