Cell Compartment Tracing using Lentiviral Fluorescent Protein Reporter Vectors

• Green Fluorescent Protein
• Red Fluorescent Protein

Accurately mark organelles and follow their dynamics with GFP/RFP-fusion tracer proteins expressed from integrated lentivectors

• Stable lentivector-based system for cell tracing
• Ideal for co-localization studies with other proteins
• Monitor protein trafficking in real-time
• Constructs for organelles, vesicles and compartments
• Bright and photostable GFP or RFP is non-disruptive to cells

Molecular trafficking is a dynamic process in eukaryotic cells. The Cyto-Tracers™ provide the ability to light up cell compartments for monitoring movement and localization of organelles and tracing endocytosis and exocytosis.
Our partner SBI has created a line of lentivector-based Cyto-Tracers™ that utilize GFP- or RFP-fusion proteins to mark cellular compartments, organelles, vesicles and structures to enable long-term and in-depth experimentation. The Cyto-Tracers can be used in transfections as well as packaged into virus to create stable GFP/RFP tracer cell lines in primary cells, tumor cell lines and stem cells.



MSCV or CMV Promoter
To ensure the expression of these constructs in a variety of cell types, constructs are available with two extensively tested promoters:
The Murine Stem Cell Virus promoter (MSCV) is useful for stem cells and hematopoietic cell types.
The Cytomegalovirus promoter (CMV)-driven constructs can be used for other, easier to infect cell types.



References
Neumann, B et al. (2010) Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 464(7289):721-7.
Eng, CH et al. (2010) Ammonia derived from glutaminolysis is a diffusible regulator of autophagy. Sci Signal. 3(119):ra31.
Löw, K et al. (2010) A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury. Gene Ther. 17(5):577-91.
Merzlyak, EM et al. (2007) Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 4(7):555-7.
Shaner, NC et al. (2007) Advances in fluorescent protein technology. J Cell Sci. 120(Pt 24):4247-60.

Vector for creating your own C-terminal fusions
Vector for creating your own N-terminal fusions

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User Manual