Encyclo PCR Kit (includes Polymerase Mix with Automated Hot Start Function)

Cat#:	PK001-EV
Quantity:	100 rxns (50 ul each)
Price:	235 €
Supplier:	Evrogen

Encyclo PCR kit is intended for most PCR and primer extension applications.
It is especially recommended for cDNA amplification due to optimal combination of high fidelity and processivity provided by Encyclo polymerase mix.

Evrogen Encyclo polymerase mix produces high yields of PCR products from a wide variety of templates and is suitable for difficult templates, long PCR (up to 15 kb), and cloning.


Encyclo polymerase mix features:

* 5’>3’ DNA polymerase activity with high processivity

* Proofreading 3’>5’ exonuclease activity

* Automatic hot start

* TA cloning compatibility

* Recommended for MINT Full Length Enriched cDNA Synthesis and TRIMMER cDNA normalization


Encyclo buffer has been developed to facilitate the amplification of specific PCR products and provide successful amplification of long DNA templates. Encyclo PCR kit includes a mix of high-purity deoxyribonucleotides as well as sterile PCR water.
Control DNA template and primer mix enclosed can be used for positive control PCR reaction.

Figure 1 demonstrates successful performance of the Encyclo PCR kit for amplification of long DNA templates. PCR amplification of total cDNA is shown in Figure 2.











Figure 1. PCR amplification of phage ë DNA fragments (lanes 1-5: 2.9 kb, 5 kb, 8 kb, 10 kb and 15 kb) using "Encyclo PCR kit". M: 50 ng of DNA size markers (1 kb DNA ladder).

















Figure 2. PCR amplification of total cDNA from different tissues: lane 1 - human skeletal muscle; lane 2 - human lung; lane 3 - human leucocytes; lane 4 - mouse brain; M: 50 ng of DNA size marker (1 kb DNA ladder).






Product Use Limitations: Encyclo PCR kit is intended for research purposes only.

Endnotes: PCR is the subject of patents issued in certain countries. The purchase of this kit does not include a license to perform PCR. However, many researchers may not be required to obtain a license. Other investigators may already have a license to perform PCR through use of a thermal cycler with the appropriate label license.

MINT Full Length Enriched cDNA Synthesis
TRIMMER cDNA Normalization

User Manual